Xendorphin B1, a novel opioid-like peptide determined from a Xenopus laevis brain cDNA library, produces opioid antinociception after spinal administration in amphibians

Craig Stevens, Géza Tóth, Anna Borsodi, Sándor Benyhe

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Prodynorphins (PDYNs) from the African clawed frog (Xenopus leavis), originally described as 'proxendorphins', are novel members of the family of opioid-like precursor polypeptides and were recently discovered based on polymerase chain reaction (PCR) isolates from a Xenopus brain cDNA library. This amphibian prodynorphin was found in two isoforms, Xen PDYN-A and Xen PDYN-B, consisting of 247 and 279 amino acids, respectively. Each prepropeptide contains five potential opioid-like peptides, collectively named xendorphins. One of these, xendorphin B1 ( Xen PDYN-B sequence 96-111: YGGFIRKPDKYKFLNA), is a hexadecapeptide that displaced [ 3 H]naloxone and the radiolabelled kappa opioid, [ 3 H]dynorphin A (1-17), with nanomolar affinity from rat brain membranes. Using the acetic acid pain test, the present study examined the antinociceptive effects of spinally administered xendorphin B1 in amphibians. Xendorphin B1 produced a long-lasting and dose-dependent antinociceptive effect in the Northern grass frog (Rana pipiens) with an ED 50 value of 44.5 nmol/frog. The antinociceptive effects of xendorphin B1 were significantly blocked by pretreatment with the non-selective opioid antagonist, naltrexone. This is the first report of the in vivo characterization of a non-mammalian prodynorphin-derived peptide and suggests that xendorphin peptides may play a role in the modulation of noxious information in vertebrates.

Original languageEnglish
Pages (from-to)628-632
Number of pages5
JournalBrain Research Bulletin
Volume71
Issue number6
DOIs
StatePublished - 30 Mar 2007

Fingerprint

Opioid Peptides
Xenopus laevis
Amphibians
Gene Library
Opioid Analgesics
Brain
Anura
Xenopus
Peptides
Rana pipiens
Dynorphins
Naltrexone
Narcotic Antagonists
Naloxone
Poaceae
Acetic Acid
Vertebrates
preproenkephalin
Xenopus Xen-dorphin prohormone
Protein Isoforms

Keywords

  • Antinociception
  • Frog
  • Opioid peptides
  • Prodynorphin
  • Rana pipiens
  • Xenopus laevis

Cite this

@article{738c3ec53ec243a89da01a7992fc3915,
title = "Xendorphin B1, a novel opioid-like peptide determined from a Xenopus laevis brain cDNA library, produces opioid antinociception after spinal administration in amphibians",
abstract = "Prodynorphins (PDYNs) from the African clawed frog (Xenopus leavis), originally described as 'proxendorphins', are novel members of the family of opioid-like precursor polypeptides and were recently discovered based on polymerase chain reaction (PCR) isolates from a Xenopus brain cDNA library. This amphibian prodynorphin was found in two isoforms, Xen PDYN-A and Xen PDYN-B, consisting of 247 and 279 amino acids, respectively. Each prepropeptide contains five potential opioid-like peptides, collectively named xendorphins. One of these, xendorphin B1 ( Xen PDYN-B sequence 96-111: YGGFIRKPDKYKFLNA), is a hexadecapeptide that displaced [ 3 H]naloxone and the radiolabelled kappa opioid, [ 3 H]dynorphin A (1-17), with nanomolar affinity from rat brain membranes. Using the acetic acid pain test, the present study examined the antinociceptive effects of spinally administered xendorphin B1 in amphibians. Xendorphin B1 produced a long-lasting and dose-dependent antinociceptive effect in the Northern grass frog (Rana pipiens) with an ED 50 value of 44.5 nmol/frog. The antinociceptive effects of xendorphin B1 were significantly blocked by pretreatment with the non-selective opioid antagonist, naltrexone. This is the first report of the in vivo characterization of a non-mammalian prodynorphin-derived peptide and suggests that xendorphin peptides may play a role in the modulation of noxious information in vertebrates.",
keywords = "Antinociception, Frog, Opioid peptides, Prodynorphin, Rana pipiens, Xenopus laevis",
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Xendorphin B1, a novel opioid-like peptide determined from a Xenopus laevis brain cDNA library, produces opioid antinociception after spinal administration in amphibians. / Stevens, Craig; Tóth, Géza; Borsodi, Anna; Benyhe, Sándor.

In: Brain Research Bulletin, Vol. 71, No. 6, 30.03.2007, p. 628-632.

Research output: Contribution to journalArticle

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T1 - Xendorphin B1, a novel opioid-like peptide determined from a Xenopus laevis brain cDNA library, produces opioid antinociception after spinal administration in amphibians

AU - Stevens, Craig

AU - Tóth, Géza

AU - Borsodi, Anna

AU - Benyhe, Sándor

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