@inbook{1129b575d8564b4a8d496e4e2e4bd3aa,
title = "Visualization of gram-positive bacterial pili",
abstract = "Pili or fimbriae are recognized as essential virulence determinants assembled on the bacterial surface. Gram-positive bacteria produce covalently linked pilus structures that are distinct from gram-negative counterparts. In this chapter, we describe three commonly used techniques to extract, detect, and visualize pili from gram-positive bacteria: (1) Western blot analysis, (2) Immuno-Electron Microscopy, and (3) Atomic Force Microscopy.",
keywords = "Atomic force microscopy, Fimbriae, Gram-positive bacteria, Immunoelectron microscopy, Pilus detection, Sortase, Western blots",
author = "Chungyu Chang and Huang, {I. Hsiu} and Hendrickx, {Antoni P.A.} and Hung Ton-That",
year = "2013",
doi = "10.1007/978-1-62703-245-2_5",
language = "English",
isbn = "9781627032445",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "77--95",
booktitle = "Bacterial Cell Surfaces",
}