Venom:antivenom immune complex binding assay using size-exclusion high-performance liquid chromatography (SE-HPLC)

Charles Sanny, Crystal A. Shults

Research output: Contribution to conferencePoster

Abstract

The treatment of envenomation with effective snake antivenom immunoglobins has become a critical worldwide health issue. Current methods for testing the effectiveness of new antivenom mixtures in neutralizing venom toxicity/lethality use animal models (e.g. mice). Neutralization of venom toxicity/lethality requires the formation of venom-antivenom immune complexes (though the extent of complex formation in vivo is unknown). Size-exclusion high-performance liquid chromatography (SE-HPLC) is a reproducible quantitative method to characterize venom-antivenom immune complex formation in vitro within a relatively short time. Changes in SE-HPLC elution profiles due to dose-dependent formation of venom-antivenom immune complexes are present for 1) Crotalis atrox (western diamondback rattlesnake) venom and the current antivenom used clinically in North America [FabAV (Ovine); CroFab™], and 2) Bothrops jararaca venom (Brazil) and Bothropic antivenom [F(ab')2AV (Equine); Brazil]. Changes in profile region areas were fit to a hyperbolic dose-response function to estimate maximum binding and venom/antivenom concentrations at half-maximum binding.
Original languageAmerican English
StatePublished - 22 Aug 2020
EventOklahoma State University Center for Health Sciences Research Day 2019 - Oklahoma State University Center for Health Sciences, TULSA, United States
Duration: 21 Feb 201922 Feb 2019
https://hdl.handle.net/11244/323882

Conference

ConferenceOklahoma State University Center for Health Sciences Research Day 2019
Abbreviated titleResearch Day 2019
CountryUnited States
CityTULSA
Period21/02/1922/02/19
Internet address

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    Sanny, C., & Shults, C. A. (2020). Venom:antivenom immune complex binding assay using size-exclusion high-performance liquid chromatography (SE-HPLC). Poster session presented at Oklahoma State University Center for Health Sciences Research Day 2019, TULSA, United States. https://shareok.org/handle/11244/323865