Abstract
The treatment of envenomation with effective snake antivenom immunoglobins has become a critical worldwide health issue. Current methods for testing the effectiveness of new antivenom mixtures in neutralizing venom toxicity/lethality use animal models (e.g. mice). Neutralization of venom toxicity/lethality requires the formation of venom-antivenom immune complexes (though the extent of complex formation in vivo is unknown). Size-exclusion high-performance liquid chromatography (SE-HPLC) is a reproducible quantitative method to characterize venom-antivenom immune complex formation in vitro within a relatively short time. Changes in SE-HPLC elution profiles due to dose-dependent formation of venom-antivenom immune complexes are present for 1) Crotalis atrox (western diamondback rattlesnake) venom and the current antivenom used clinically in North America [FabAV (Ovine); CroFab™], and 2) Bothrops jararaca venom (Brazil) and Bothropic antivenom [F(ab')2AV (Equine); Brazil]. Changes in profile region areas were fit to a hyperbolic dose-response function to estimate maximum binding and venom/antivenom concentrations at half-maximum binding.
| Original language | American English |
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| State | Published - 22 Aug 2020 |
| Event | Oklahoma State University Center for Health Sciences Research Day 2019 - Oklahoma State University Center for Health Sciences, TULSA, United States Duration: 21 Feb 2019 → 22 Feb 2019 https://openresearch.okstate.edu/handle/20.500.14446/323834 (Open Research Oklahoma - OSU Center for Health Sciences - Research Day 2019) |
Conference
| Conference | Oklahoma State University Center for Health Sciences Research Day 2019 |
|---|---|
| Abbreviated title | Research Day 2019 |
| Country/Territory | United States |
| City | TULSA |
| Period | 21/02/19 → 22/02/19 |
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