Use of mini-columns packed with acid-washed florisil for the rapid separation of A, E and F series prostaglandins

Martin W. Banschbach, Paula K. Love

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

A new procedure was developed for the rapid isolation of the A, E and F series prostaglandins from plasma extracts. This new procedure involves the use of mini-columns packed with acid-washed Florisil to rapidly separate the A, E and F series prostaglandins. These new mini-columns give flow rates of around 1 ml/min. and they generate a well-resolved chromatographic pattern while at the same time producing good recoveries for [3H]-labeled prostaglandins of the A, E and F series. Using these new mini-columns, large numbers of plasma extracts can be processed in a short period of time.

Original languageEnglish
Pages (from-to)193-200
Number of pages8
JournalProstaglandins
Volume17
Issue number2
DOIs
Publication statusPublished - Feb 1979

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