Use of [1-14C]acetic anhydride to quantitate diglycerides: A new analytical procedure

M. W. Banschbach; R. L. Geison; J. F. O'Brien

doi:10.1016/0003-2697(74)90315-7

Use of [1-¹⁴C]acetic anhydride to quantitate diglycerides: A new analytical procedure

M. W. Banschbach, R. L. Geison, J. F. O'Brien

Biochemistry & Microbiology

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18 Scopus citations

Abstract

A microanalytical procedure is described for perchloric acid-catalyzed acetylation of diglycerides in pyridine using [1-¹⁴C]acetic anhydride of known specific activity. Incorporated [1-¹⁴C]acetate permits the rapid, accurate, and nondestructive quantitation of diglycerides in the concentration range of 0.005 μmoles to 1 μmole. Diglyceride is completely acetylated within 20-25 min at room temperature. No intermolecular rearrangements are observed; some intramolecular rearrangement does occur.

Original language	English
Pages (from-to)	617-627
Number of pages	11
Journal	Analytical Biochemistry
Volume	59
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(74)90315-7
State	Published - Jun 1974

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title = "Use of [1-14C]acetic anhydride to quantitate diglycerides: A new analytical procedure",

abstract = "A microanalytical procedure is described for perchloric acid-catalyzed acetylation of diglycerides in pyridine using [1-14C]acetic anhydride of known specific activity. Incorporated [1-14C]acetate permits the rapid, accurate, and nondestructive quantitation of diglycerides in the concentration range of 0.005 μmoles to 1 μmole. Diglyceride is completely acetylated within 20-25 min at room temperature. No intermolecular rearrangements are observed; some intramolecular rearrangement does occur.",

author = "Banschbach, \{M. W.\} and Geison, \{R. L.\} and O'Brien, \{J. F.\}",

year = "1974",

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T1 - Use of [1-14C]acetic anhydride to quantitate diglycerides

T2 - A new analytical procedure

AU - Banschbach, M. W.

AU - Geison, R. L.

AU - O'Brien, J. F.

PY - 1974/6

Y1 - 1974/6

N2 - A microanalytical procedure is described for perchloric acid-catalyzed acetylation of diglycerides in pyridine using [1-14C]acetic anhydride of known specific activity. Incorporated [1-14C]acetate permits the rapid, accurate, and nondestructive quantitation of diglycerides in the concentration range of 0.005 μmoles to 1 μmole. Diglyceride is completely acetylated within 20-25 min at room temperature. No intermolecular rearrangements are observed; some intramolecular rearrangement does occur.

AB - A microanalytical procedure is described for perchloric acid-catalyzed acetylation of diglycerides in pyridine using [1-14C]acetic anhydride of known specific activity. Incorporated [1-14C]acetate permits the rapid, accurate, and nondestructive quantitation of diglycerides in the concentration range of 0.005 μmoles to 1 μmole. Diglyceride is completely acetylated within 20-25 min at room temperature. No intermolecular rearrangements are observed; some intramolecular rearrangement does occur.

UR - https://www.scopus.com/pages/publications/0016077092

U2 - 10.1016/0003-2697(74)90315-7

DO - 10.1016/0003-2697(74)90315-7

M3 - Article

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AN - SCOPUS:0016077092

SN - 0003-2697

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EP - 627

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 2

ER -

Use of [1-¹⁴C]acetic anhydride to quantitate diglycerides: A new analytical procedure

Abstract

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