Use of [1-14C]acetic anhydride to quantitate diglycerides: A new analytical procedure

Martin Banschbach, R. L. Geison, J. F. O'Brien

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

A microanalytical procedure is described for perchloric acid-catalyzed acetylation of diglycerides in pyridine using [1-14C]acetic anhydride of known specific activity. Incorporated [1-14C]acetate permits the rapid, accurate, and nondestructive quantitation of diglycerides in the concentration range of 0.005 μmoles to 1 μmole. Diglyceride is completely acetylated within 20-25 min at room temperature. No intermolecular rearrangements are observed; some intramolecular rearrangement does occur.

Original languageEnglish
Pages (from-to)617-627
Number of pages11
JournalAnalytical Biochemistry
Volume59
Issue number2
DOIs
StatePublished - 1 Jan 1974

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Diglycerides
Acetylation
Acetates
Temperature
acetic anhydride

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Use of [1-14C]acetic anhydride to quantitate diglycerides : A new analytical procedure. / Banschbach, Martin; Geison, R. L.; O'Brien, J. F.

In: Analytical Biochemistry, Vol. 59, No. 2, 01.01.1974, p. 617-627.

Research output: Contribution to journalArticle

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