A microanalytical procedure is described for perchloric acid-catalyzed acetylation of diglycerides in pyridine using [1-14C]acetic anhydride of known specific activity. Incorporated [1-14C]acetate permits the rapid, accurate, and nondestructive quantitation of diglycerides in the concentration range of 0.005 μmoles to 1 μmole. Diglyceride is completely acetylated within 20-25 min at room temperature. No intermolecular rearrangements are observed; some intramolecular rearrangement does occur.
|Number of pages||11|
|State||Published - Jun 1974|