Use of [1-14C]acetic anhydride to quantitate diglycerides: A new analytical procedure

M. W. Banschbach; R. L. Geison; J. F. O'Brien

doi:10.1016/0003-2697(74)90315-7

Use of [1-¹⁴C]acetic anhydride to quantitate diglycerides: A new analytical procedure

M. W. Banschbach, R. L. Geison, J. F. O'Brien

Biochemistry & Microbiology

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

A microanalytical procedure is described for perchloric acid-catalyzed acetylation of diglycerides in pyridine using [1-¹⁴C]acetic anhydride of known specific activity. Incorporated [1-¹⁴C]acetate permits the rapid, accurate, and nondestructive quantitation of diglycerides in the concentration range of 0.005 μmoles to 1 μmole. Diglyceride is completely acetylated within 20-25 min at room temperature. No intermolecular rearrangements are observed; some intramolecular rearrangement does occur.

Original language	English
Pages (from-to)	617-627
Number of pages	11
Journal	Analytical Biochemistry
Volume	59
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(74)90315-7
State	Published - Jun 1974

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abstract = "A microanalytical procedure is described for perchloric acid-catalyzed acetylation of diglycerides in pyridine using [1-14C]acetic anhydride of known specific activity. Incorporated [1-14C]acetate permits the rapid, accurate, and nondestructive quantitation of diglycerides in the concentration range of 0.005 μmoles to 1 μmole. Diglyceride is completely acetylated within 20-25 min at room temperature. No intermolecular rearrangements are observed; some intramolecular rearrangement does occur.",

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AB - A microanalytical procedure is described for perchloric acid-catalyzed acetylation of diglycerides in pyridine using [1-14C]acetic anhydride of known specific activity. Incorporated [1-14C]acetate permits the rapid, accurate, and nondestructive quantitation of diglycerides in the concentration range of 0.005 μmoles to 1 μmole. Diglyceride is completely acetylated within 20-25 min at room temperature. No intermolecular rearrangements are observed; some intramolecular rearrangement does occur.

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ER -

Use of [1-¹⁴C]acetic anhydride to quantitate diglycerides: A new analytical procedure

Abstract

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