Use of [1-14C]acetic anhydride to quantitate diglycerides: A new analytical procedure

M. W. Banschbach, R. L. Geison, J. F. O'Brien

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A microanalytical procedure is described for perchloric acid-catalyzed acetylation of diglycerides in pyridine using [1-14C]acetic anhydride of known specific activity. Incorporated [1-14C]acetate permits the rapid, accurate, and nondestructive quantitation of diglycerides in the concentration range of 0.005 μmoles to 1 μmole. Diglyceride is completely acetylated within 20-25 min at room temperature. No intermolecular rearrangements are observed; some intramolecular rearrangement does occur.

Original languageEnglish
Pages (from-to)617-627
Number of pages11
JournalAnalytical Biochemistry
Issue number2
Publication statusPublished - Jun 1974


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