Tissue and species distribution of mRNA for the I(kr)-like K+ channel, erg

Randy Wymore, Gary A. Gintant, Rigel T. Wymore, Jane E. Dixon, David McKinnon, Ira S. Cohen

Research output: Contribution to journalArticle

139 Citations (Scopus)

Abstract

The human K+ channel gene, HERG, has been linked to the type 2 form of the autosomal dominant long-QT syndrome and has been suggested to encode the fast component of the delayed rectifier K+ current (I(Kr)) found in heart. To date, the published electrophysiological and pharmacological data on the Xenopus expressed HERG are very similar but are not identical to those of the endogenous I(Kr). In an effort to provide a different type of correlative data on the relationship between erg and I(Kr), cDNA fragments of erg homologues from guinea pig, rabbit, human, dog, and rat were cloned and used to test for the presence of erg mRNA in cardiac tissue. RNase protection assays reveal that erg message is found in the hearts of all five species and that it is expressed uniformly throughout the heart. The erg transcript is expressed at relatively high levels, being ≃50% more abundant than the most prevalent Kv-class K+ channel transcript in canine ventricle (Kv4.3), erg transcripts were found to have a wide tissue distribution in rat and are abundant in the brain, retina, thymus, and adrenal gland and are also found in skeletal muscle, lung, and cornea. Since there were no published reports of an I(Kr)-like current in the rat heart, electrophysiological studies were performed to test whether the significant level of erg message in rat heart was correlated with the presence of an I(Kr)-like current in rat. In isolated rat ventricular myocytes, an E-4031-sensitive current was observed, which is consistent with the presence of I(Kr). These results strengthen the link between erg and the native I(Kr) in heart and suggest that erg may play an important role in other noncardiac tissues.

Original languageEnglish
Pages (from-to)261-268
Number of pages8
JournalCirculation Research
Volume80
Issue number2
DOIs
StatePublished - 1 Jan 1997

Fingerprint

Tissue Distribution
Messenger RNA
Long QT Syndrome
Ribonucleases
Adrenal Glands
Xenopus
Cornea
Muscle Cells
Thymus Gland
Retina
Canidae
Guinea Pigs
Skeletal Muscle
Complementary DNA
Pharmacology
Dogs
Rabbits
Lung
Brain
Genes

Keywords

  • K channel
  • cardiac muscle
  • delayed rectifier K current
  • mRNA expression

Cite this

Wymore, R., Gintant, G. A., Wymore, R. T., Dixon, J. E., McKinnon, D., & Cohen, I. S. (1997). Tissue and species distribution of mRNA for the I(kr)-like K+ channel, erg. Circulation Research, 80(2), 261-268. https://doi.org/10.1161/01.RES.80.2.261
Wymore, Randy ; Gintant, Gary A. ; Wymore, Rigel T. ; Dixon, Jane E. ; McKinnon, David ; Cohen, Ira S. / Tissue and species distribution of mRNA for the I(kr)-like K+ channel, erg. In: Circulation Research. 1997 ; Vol. 80, No. 2. pp. 261-268.
@article{f0d56b30e6d44792a74914066ee11ee7,
title = "Tissue and species distribution of mRNA for the I(kr)-like K+ channel, erg",
abstract = "The human K+ channel gene, HERG, has been linked to the type 2 form of the autosomal dominant long-QT syndrome and has been suggested to encode the fast component of the delayed rectifier K+ current (I(Kr)) found in heart. To date, the published electrophysiological and pharmacological data on the Xenopus expressed HERG are very similar but are not identical to those of the endogenous I(Kr). In an effort to provide a different type of correlative data on the relationship between erg and I(Kr), cDNA fragments of erg homologues from guinea pig, rabbit, human, dog, and rat were cloned and used to test for the presence of erg mRNA in cardiac tissue. RNase protection assays reveal that erg message is found in the hearts of all five species and that it is expressed uniformly throughout the heart. The erg transcript is expressed at relatively high levels, being ≃50{\%} more abundant than the most prevalent Kv-class K+ channel transcript in canine ventricle (Kv4.3), erg transcripts were found to have a wide tissue distribution in rat and are abundant in the brain, retina, thymus, and adrenal gland and are also found in skeletal muscle, lung, and cornea. Since there were no published reports of an I(Kr)-like current in the rat heart, electrophysiological studies were performed to test whether the significant level of erg message in rat heart was correlated with the presence of an I(Kr)-like current in rat. In isolated rat ventricular myocytes, an E-4031-sensitive current was observed, which is consistent with the presence of I(Kr). These results strengthen the link between erg and the native I(Kr) in heart and suggest that erg may play an important role in other noncardiac tissues.",
keywords = "K channel, cardiac muscle, delayed rectifier K current, mRNA expression",
author = "Randy Wymore and Gintant, {Gary A.} and Wymore, {Rigel T.} and Dixon, {Jane E.} and David McKinnon and Cohen, {Ira S.}",
year = "1997",
month = "1",
day = "1",
doi = "10.1161/01.RES.80.2.261",
language = "English",
volume = "80",
pages = "261--268",
journal = "Circulation research",
issn = "0009-7330",
publisher = "Lippincott Williams and Wilkins Ltd.",
number = "2",

}

Wymore, R, Gintant, GA, Wymore, RT, Dixon, JE, McKinnon, D & Cohen, IS 1997, 'Tissue and species distribution of mRNA for the I(kr)-like K+ channel, erg', Circulation Research, vol. 80, no. 2, pp. 261-268. https://doi.org/10.1161/01.RES.80.2.261

Tissue and species distribution of mRNA for the I(kr)-like K+ channel, erg. / Wymore, Randy; Gintant, Gary A.; Wymore, Rigel T.; Dixon, Jane E.; McKinnon, David; Cohen, Ira S.

In: Circulation Research, Vol. 80, No. 2, 01.01.1997, p. 261-268.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Tissue and species distribution of mRNA for the I(kr)-like K+ channel, erg

AU - Wymore, Randy

AU - Gintant, Gary A.

AU - Wymore, Rigel T.

AU - Dixon, Jane E.

AU - McKinnon, David

AU - Cohen, Ira S.

PY - 1997/1/1

Y1 - 1997/1/1

N2 - The human K+ channel gene, HERG, has been linked to the type 2 form of the autosomal dominant long-QT syndrome and has been suggested to encode the fast component of the delayed rectifier K+ current (I(Kr)) found in heart. To date, the published electrophysiological and pharmacological data on the Xenopus expressed HERG are very similar but are not identical to those of the endogenous I(Kr). In an effort to provide a different type of correlative data on the relationship between erg and I(Kr), cDNA fragments of erg homologues from guinea pig, rabbit, human, dog, and rat were cloned and used to test for the presence of erg mRNA in cardiac tissue. RNase protection assays reveal that erg message is found in the hearts of all five species and that it is expressed uniformly throughout the heart. The erg transcript is expressed at relatively high levels, being ≃50% more abundant than the most prevalent Kv-class K+ channel transcript in canine ventricle (Kv4.3), erg transcripts were found to have a wide tissue distribution in rat and are abundant in the brain, retina, thymus, and adrenal gland and are also found in skeletal muscle, lung, and cornea. Since there were no published reports of an I(Kr)-like current in the rat heart, electrophysiological studies were performed to test whether the significant level of erg message in rat heart was correlated with the presence of an I(Kr)-like current in rat. In isolated rat ventricular myocytes, an E-4031-sensitive current was observed, which is consistent with the presence of I(Kr). These results strengthen the link between erg and the native I(Kr) in heart and suggest that erg may play an important role in other noncardiac tissues.

AB - The human K+ channel gene, HERG, has been linked to the type 2 form of the autosomal dominant long-QT syndrome and has been suggested to encode the fast component of the delayed rectifier K+ current (I(Kr)) found in heart. To date, the published electrophysiological and pharmacological data on the Xenopus expressed HERG are very similar but are not identical to those of the endogenous I(Kr). In an effort to provide a different type of correlative data on the relationship between erg and I(Kr), cDNA fragments of erg homologues from guinea pig, rabbit, human, dog, and rat were cloned and used to test for the presence of erg mRNA in cardiac tissue. RNase protection assays reveal that erg message is found in the hearts of all five species and that it is expressed uniformly throughout the heart. The erg transcript is expressed at relatively high levels, being ≃50% more abundant than the most prevalent Kv-class K+ channel transcript in canine ventricle (Kv4.3), erg transcripts were found to have a wide tissue distribution in rat and are abundant in the brain, retina, thymus, and adrenal gland and are also found in skeletal muscle, lung, and cornea. Since there were no published reports of an I(Kr)-like current in the rat heart, electrophysiological studies were performed to test whether the significant level of erg message in rat heart was correlated with the presence of an I(Kr)-like current in rat. In isolated rat ventricular myocytes, an E-4031-sensitive current was observed, which is consistent with the presence of I(Kr). These results strengthen the link between erg and the native I(Kr) in heart and suggest that erg may play an important role in other noncardiac tissues.

KW - K channel

KW - cardiac muscle

KW - delayed rectifier K current

KW - mRNA expression

UR - http://www.scopus.com/inward/record.url?scp=0031028568&partnerID=8YFLogxK

U2 - 10.1161/01.RES.80.2.261

DO - 10.1161/01.RES.80.2.261

M3 - Article

C2 - 9012748

AN - SCOPUS:0031028568

VL - 80

SP - 261

EP - 268

JO - Circulation research

JF - Circulation research

SN - 0009-7330

IS - 2

ER -