The effects of laser immunotherapy on cancer cell migration

Cody F. Bahavar, Feifan Zhou, Aamr M. Hasanjee, Elivia Layton, Anh Lam, Wei R. Chen, Melville B. Vaughan

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Laser immunotherapy (LIT) uses laser irradiation and immunological stimulation to target all types of metastases and creates a long-term tumor resistance. Glycated chitosan (GC) is the immunological stimulant used in LIT. Interestingly, GC can act as a surfactant for single-walled carbon nanotubes (SWNTs) to immunologically modify SWNTs. SWNT-GC retains the optical properties of SWNTs and the immunological functions of GC to help increase the selectivity of the laser and create a more optimal immune response. One essential aspect of understanding this immune response is knowing how laser irradiation affects cancer cells' ability to metastasize. In this experiment, a cell migration assay was performed. A 2mm circular elastomer plugs were placed at the bottom of multi-well dishes. Pre-cancerous keratinocytes, different tumor cells, and fibroblasts were then plated separately in treated wells. Once the cells reached 100% confluence, they were irradiated by either a 980nm or 805nm wavelength laser. The goal was to determine the effects of laser irradiation and immunological stimulation on cancer cell migration in vitro, paying the way to understand the mechanism of LIT in treating metastatic tumors in cancer patients.

Original languageEnglish
Title of host publicationBiophotonics and Immune Responses XI
EditorsWei R. Chen
PublisherSPIE
ISBN (Electronic)9781628419436
DOIs
StatePublished - 1 Jan 2016
Externally publishedYes
EventBiophotonics and Immune Responses XI - San Francisco, United States
Duration: 15 Feb 2016 → …

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume9709
ISSN (Print)1605-7422

Conference

ConferenceBiophotonics and Immune Responses XI
Country/TerritoryUnited States
CitySan Francisco
Period15/02/16 → …

Keywords

  • Cell migration
  • fibroblasts
  • glycated chitosan
  • immunological stimulant
  • keratinocytes
  • laser irradiation
  • metastatic cancer

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