The cytoskeleton of cultured skin fibroblasts from patients with Huntington's chorea

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    Abstract

    Cultured skin fibroblasts from patients with Huntington's chorea were prepared for indirect immunofluorescence using monospecific antibodies to tubulin, actin, and fibronectin. The fibroblasts were also visualized by transmission electron microscopy. The fibroblasts were observed after 3 hours of plating and treatment with various concentrations of colcemid and cytochalasin B to test the reaction of the microfibrillar network to stressful conditions. Disorders were not apparent in the cytoskeletal system (microtubules, microfilaments, and intermediate filaments) when compared with normal controls. Fibronectin was arranged in a fibrillar pattern similar to that seen with actin immunofluorescence. This colinear arrangement was not disturbed in Huntington's chorea cells. Microtubules, microfilaments, and 10-nm intermediate filaments became more parallel as the incubation period increased from 3 to 24 hours. This study showed that the cytoskeleton and the attachment of one surface protein (fibronectin) are not affected in Huntington' chorea.

    Original languageEnglish
    Pages (from-to)241-253
    Number of pages13
    JournalJournal of the American Osteopathic Association
    Volume90
    Issue number3
    StatePublished - 1 Jan 1990

    Fingerprint

    Huntington Disease
    Cytoskeleton
    Fibronectins
    Intermediate Filaments
    Fibroblasts
    Actin Cytoskeleton
    Microtubules
    Skin
    Actins
    Demecolcine
    Cytochalasin B
    Tubulin
    Indirect Fluorescent Antibody Technique
    Transmission Electron Microscopy
    Fluorescent Antibody Technique
    Membrane Proteins
    Antibodies
    Therapeutics

    Cite this

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    title = "The cytoskeleton of cultured skin fibroblasts from patients with Huntington's chorea",
    abstract = "Cultured skin fibroblasts from patients with Huntington's chorea were prepared for indirect immunofluorescence using monospecific antibodies to tubulin, actin, and fibronectin. The fibroblasts were also visualized by transmission electron microscopy. The fibroblasts were observed after 3 hours of plating and treatment with various concentrations of colcemid and cytochalasin B to test the reaction of the microfibrillar network to stressful conditions. Disorders were not apparent in the cytoskeletal system (microtubules, microfilaments, and intermediate filaments) when compared with normal controls. Fibronectin was arranged in a fibrillar pattern similar to that seen with actin immunofluorescence. This colinear arrangement was not disturbed in Huntington's chorea cells. Microtubules, microfilaments, and 10-nm intermediate filaments became more parallel as the incubation period increased from 3 to 24 hours. This study showed that the cytoskeleton and the attachment of one surface protein (fibronectin) are not affected in Huntington' chorea.",
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    The cytoskeleton of cultured skin fibroblasts from patients with Huntington's chorea. / Meek, W. D.

    In: Journal of the American Osteopathic Association, Vol. 90, No. 3, 01.01.1990, p. 241-253.

    Research output: Contribution to journalArticle

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