Subcellular distribution of Plp-40, a lipoprotein in a serotype A strain of Pasteurella multocida

Margaret N. Nsofor, Phillip E. Ryals, Franklin Champlin

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A 40-kDa lipoprotein (Plp-40) is expressed by serotype A strains of Pasteurella multocida in amounts which correlate with the amount of capsular material present. We hypothesized that Plp-40 is exposed at the outer surface of the outer membrane (OM) of the cell and is associated with the serotype A exopolysaccharide material. The objectives of the present study were to confirm the lipoprotein nature of Plp-40 and to determine its subcellular location. Plp-40 maturation was shown to be sensitive to globomycin, thereby confirming it to be a bacterial lipoprotein. Plp-40 was shown to be present in the OM fractions of P. multocida obtained by both sarkosyl extraction and sucrose density gradient centrifugation, as well as in capsule fractions obtained by either hyaluronidase treatment or warm buffer extraction. [3H]palmitic acid-labeled Plp-40 could be removed from the surface of whole cells by exposure to proteinase K. Autoradiography of 125I-labeled cell surface proteins exhibited a 40-kDa band that was prominent in capsulated strains and greatly diminished in a noncapsulated strain. These results support the hypothesis that Plp-40 is a lipid-modified OM protein, which is exposed on the outer cell surface and is likely associated with serotype A extracellular polysaccharide.

Original languageEnglish
Pages (from-to)1160-1166
Number of pages7
JournalBiochimica et Biophysica Acta - General Subjects
Volume1760
Issue number8
DOIs
StatePublished - 1 Aug 2006

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Pasteurella multocida
Lipoproteins
Membrane Proteins
Membranes
Endopeptidase K
Hyaluronoglucosaminidase
Density Gradient Centrifugation
Palmitic Acid
Centrifugation
Autoradiography
Capsules
Polysaccharides
Sucrose
Buffers
Cell Membrane
Lipids
Serogroup

Keywords

  • Capsule
  • Cell envelope
  • Outer membrane lipoprotein
  • Pasteurella multocida

Cite this

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title = "Subcellular distribution of Plp-40, a lipoprotein in a serotype A strain of Pasteurella multocida",
abstract = "A 40-kDa lipoprotein (Plp-40) is expressed by serotype A strains of Pasteurella multocida in amounts which correlate with the amount of capsular material present. We hypothesized that Plp-40 is exposed at the outer surface of the outer membrane (OM) of the cell and is associated with the serotype A exopolysaccharide material. The objectives of the present study were to confirm the lipoprotein nature of Plp-40 and to determine its subcellular location. Plp-40 maturation was shown to be sensitive to globomycin, thereby confirming it to be a bacterial lipoprotein. Plp-40 was shown to be present in the OM fractions of P. multocida obtained by both sarkosyl extraction and sucrose density gradient centrifugation, as well as in capsule fractions obtained by either hyaluronidase treatment or warm buffer extraction. [3H]palmitic acid-labeled Plp-40 could be removed from the surface of whole cells by exposure to proteinase K. Autoradiography of 125I-labeled cell surface proteins exhibited a 40-kDa band that was prominent in capsulated strains and greatly diminished in a noncapsulated strain. These results support the hypothesis that Plp-40 is a lipid-modified OM protein, which is exposed on the outer cell surface and is likely associated with serotype A extracellular polysaccharide.",
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Subcellular distribution of Plp-40, a lipoprotein in a serotype A strain of Pasteurella multocida. / Nsofor, Margaret N.; Ryals, Phillip E.; Champlin, Franklin.

In: Biochimica et Biophysica Acta - General Subjects, Vol. 1760, No. 8, 01.08.2006, p. 1160-1166.

Research output: Contribution to journalArticle

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