TY - JOUR
T1 - Stereoselective RP-HPLC determination of esmolol enantiomers in human plasma after pre-column derivatization
AU - Tang, Yi Hong
AU - He, Ying
AU - Yao, Tong Wei
AU - Zeng, Su
N1 - Funding Information:
This project was supported by Natural Science Foundation of China (30225047,39770868), and sponsored by SRF for ROCS, SEM and Zhejiang Provincial Natural Science Foundation of China (#RC97016).
PY - 2004/5/31
Y1 - 2004/5/31
N2 - A stereoselective reversed-phase HPLC assay to determine S-(-) and R-(+) enantiomers of esmolol in human plasma was developed. The method involved liquid-liquid extraction of esmolol from human plasma, using S-(-)-propranolol as the internal standard, and employed 2,3,4,6-tetra-O-acetyl-β-D- glucopyranosyl isothiocyanate as a pre-column chiral derivatization reagent. The derivatized products were separated on a 5-μm reversed-phase C18 column with a mixture of acetonitrile/0.02 mol/L phosphate buffer (pH 4.5) (55:45, v/v) as mobile phase. The detection of esmolol derivatives was made at λ=224 nm with UV detector. The assay was linear from 0.035 to 12 μg/ml for each enantiomer. The analytical method afforded average recoveries of 94.8% and 95.5% for S-(-)- and R-(+)-esmolol, respectively. For each enantiomer, the limit of detection was 0.003 μg/ml and the limit of quantification for the method was 0.035 μg/ml (RSD<14%). The reproducibility of the assay was satisfactory.
AB - A stereoselective reversed-phase HPLC assay to determine S-(-) and R-(+) enantiomers of esmolol in human plasma was developed. The method involved liquid-liquid extraction of esmolol from human plasma, using S-(-)-propranolol as the internal standard, and employed 2,3,4,6-tetra-O-acetyl-β-D- glucopyranosyl isothiocyanate as a pre-column chiral derivatization reagent. The derivatized products were separated on a 5-μm reversed-phase C18 column with a mixture of acetonitrile/0.02 mol/L phosphate buffer (pH 4.5) (55:45, v/v) as mobile phase. The detection of esmolol derivatives was made at λ=224 nm with UV detector. The assay was linear from 0.035 to 12 μg/ml for each enantiomer. The analytical method afforded average recoveries of 94.8% and 95.5% for S-(-)- and R-(+)-esmolol, respectively. For each enantiomer, the limit of detection was 0.003 μg/ml and the limit of quantification for the method was 0.035 μg/ml (RSD<14%). The reproducibility of the assay was satisfactory.
KW - Diastereoselective chromatography
KW - Enantiomer separation
KW - Esmolol
KW - RP-HPLC
UR - http://www.scopus.com/inward/record.url?scp=2442625481&partnerID=8YFLogxK
U2 - 10.1016/j.jbbm.2003.12.006
DO - 10.1016/j.jbbm.2003.12.006
M3 - Article
C2 - 15163527
AN - SCOPUS:2442625481
SN - 0165-022X
VL - 59
SP - 159
EP - 166
JO - Journal of biochemical and biophysical methods
JF - Journal of biochemical and biophysical methods
IS - 2
ER -