Status of bacterial colonization, Toll-like receptor expression and nuclear factor-kappa B activation in normal and diseased human livers

Rashmi Singh, Jay Bullard, Mamta Kalra, Senait Assefa, Anil K. Kaul, Krystal Vonfeldt, Stephen C. Strom, Robert S. Conrad, Harvey L. Sharp, Rashmi Kaul

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Epidemiological data on bacterial translocation (BT), colonization and inflammation in normal human livers is lacking. In this study we investigated the status of bacterial colonization and inflammation in the normal, cirrhotic primary biliary cirrhosis (PBC), and nonalcoholic steatohepatitis (NASH) human liver tissues. Comparatively normal livers showed increased bacterial colonization than PBC and NASH. We analyzed mRNA levels of Toll-like receptors (TLR) 2 and TLR4, and protein levels of TLR4. Phosphorylated IKKα (pIKKα) protein estimation served as a marker for nuclear factor-kappa B (NF-κB) activation. In spite of the increased bacterial colonization in normal liver tissues, lower levels of TLR2/4 mRNA and TLR4 and pIKKα proteins were found compared to PBC and NASH indicating the maintenance of suppressed inflammation and immune tolerance in normal livers. To our knowledge, this is the first clinical evidence showing suppressed inflammation despite bacterial colonization in normal human livers thus maintaining liver immune homeostasis.

Original languageEnglish
Pages (from-to)41-49
Number of pages9
JournalClinical Immunology
Volume138
Issue number1
DOIs
StatePublished - 1 Jan 2011

Fingerprint

NF-kappa B
Toll-Like Receptors
Liver Diseases
Liver
Biliary Liver Cirrhosis
Inflammation
Bacterial Translocation
Toll-Like Receptor 2
Immune Tolerance
Messenger RNA
Proteins
Homeostasis
Maintenance
Non-alcoholic Fatty Liver Disease

Keywords

  • Bacterial translocation
  • Immune homeostasis
  • Liver cirrhosis
  • Liver infection
  • Nuclear factor-kappa B
  • Toll-like receptors

Cite this

Singh, Rashmi ; Bullard, Jay ; Kalra, Mamta ; Assefa, Senait ; Kaul, Anil K. ; Vonfeldt, Krystal ; Strom, Stephen C. ; Conrad, Robert S. ; Sharp, Harvey L. ; Kaul, Rashmi. / Status of bacterial colonization, Toll-like receptor expression and nuclear factor-kappa B activation in normal and diseased human livers. In: Clinical Immunology. 2011 ; Vol. 138, No. 1. pp. 41-49.
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Status of bacterial colonization, Toll-like receptor expression and nuclear factor-kappa B activation in normal and diseased human livers. / Singh, Rashmi; Bullard, Jay; Kalra, Mamta; Assefa, Senait; Kaul, Anil K.; Vonfeldt, Krystal; Strom, Stephen C.; Conrad, Robert S.; Sharp, Harvey L.; Kaul, Rashmi.

In: Clinical Immunology, Vol. 138, No. 1, 01.01.2011, p. 41-49.

Research output: Contribution to journalArticle

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