Sqd interacts with the Drosophila retinoblastoma tumor suppressor Rbf

Joseph Ahlander, Giovanni Bosco

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The retinoblastoma tumor suppressor (RB) serves as a scaffold to coordinate binding of numerous proteins, including E2F and histone deacetylases, through its C-terminal domain. The amino-terminal half of RB has few known binding partners and its function is not well understood. We used the amino-terminal domain of the Drosophila retinoblastoma tumor suppressor Rbf (RbfN) to identify novel binding partners by immunoprecipitation coupled with mass spectrometry. Our experiment revealed that the RNA-binding protein Squid (Sqd) is a putative interacting partner of RbfN. Western blot confirmed that Sqd interacts with the amino-terminal domain of Rbf. We observed that Sqd colocalizes with RbfN in Drosophila salivary gland cells. We also show that double RNAi knockdown of Rbf and Sqd in the eye results in an extensive loss of eye bristles, suggesting that Rbf and Sqd function in a common pathway. We conclude from our studies that Rbf physically and genetically interacts with Sqd. We propose that the retinoblastoma tumor suppressor may play a novel role in RNA processing through interaction with RNA-binding proteins.

Original languageEnglish
Pages (from-to)363-367
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume383
Issue number3
DOIs
StatePublished - 5 Jun 2009
Externally publishedYes

Fingerprint

Decapodiformes
Retinoblastoma
Drosophila
Tumors
RNA-Binding Proteins
Neoplasms
Histone Deacetylases
Scaffolds
Mass spectrometry
Carrier Proteins
RNA
Processing
RNA Interference
Salivary Glands
Immunoprecipitation
Mass Spectrometry
Western Blotting
Experiments

Keywords

  • hnRNP
  • RB
  • Rbf
  • Rbf1
  • Retinoblastoma
  • RNA binding
  • RNA processing
  • Sqd
  • Tumor suppressor

Cite this

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Sqd interacts with the Drosophila retinoblastoma tumor suppressor Rbf. / Ahlander, Joseph; Bosco, Giovanni.

In: Biochemical and Biophysical Research Communications, Vol. 383, No. 3, 05.06.2009, p. 363-367.

Research output: Contribution to journalArticle

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AU - Bosco, Giovanni

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