Single‐base substitutions give rise to a five‐banded DNA profile at the D10S28 locus

Robert Allen, C. E. Sanford‐Sharp

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: DNA profiles from variable number of tandem repeat (VNTR) loci typically are composed of two bands, one derived from each member of the homologous pair of chromosomes. DNA profiles composed of more than two bands result from mutations, and the uestion arises as to the mechanism underlying these unusual multibanded DNA profiles. Study Design and Methods: An alleged father in a paternity test was found to have a five‐banded DNA profile at the D10S28 locus when his DNA was subjected to single‐locus restriction fragment length polymorphism mapping with the restriction enzyme Pvu II. Results: Several results suggest that this complex DNA profile is the result of several single change within the VNTR locus First, there was no evidence of partial digestion of the DNA with Pvu II. Furthermore, the multibanded allele happened, in this case, to be transmitted to the child, who also showed a five‐banded pattern composed of four bands inherited from the alleged father and one band inherited from the mother. Second, digestion of this DNA with Hae III resulted in the visualization of just two bands at the D10S28 locus. Conclusion: The results confirm the notion that mutations at VNTR loci are not always the result of additions or deletions of tandem repeats, but that they can also involve single‐base substitutions either within or flanking VNTR loci that give rise to atypical DNA profiles and new alleles at the locus. single‐base changes within or flanking VNTR loci that give rise to a typical DNA profiles and new alleles at the locus. 1994 AABB

Original languageEnglish
Pages (from-to)412-414
Number of pages3
JournalTransfusion
Volume34
Issue number5
DOIs
StatePublished - 1 Jan 1994

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Minisatellite Repeats
DNA
Alleles
Fathers
Digestion
Restriction Mapping
Mutation
Tandem Repeat Sequences
Restriction Fragment Length Polymorphisms
Chromosomes
Mothers

Cite this

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title = "Single‐base substitutions give rise to a five‐banded DNA profile at the D10S28 locus",
abstract = "Background: DNA profiles from variable number of tandem repeat (VNTR) loci typically are composed of two bands, one derived from each member of the homologous pair of chromosomes. DNA profiles composed of more than two bands result from mutations, and the uestion arises as to the mechanism underlying these unusual multibanded DNA profiles. Study Design and Methods: An alleged father in a paternity test was found to have a five‐banded DNA profile at the D10S28 locus when his DNA was subjected to single‐locus restriction fragment length polymorphism mapping with the restriction enzyme Pvu II. Results: Several results suggest that this complex DNA profile is the result of several single change within the VNTR locus First, there was no evidence of partial digestion of the DNA with Pvu II. Furthermore, the multibanded allele happened, in this case, to be transmitted to the child, who also showed a five‐banded pattern composed of four bands inherited from the alleged father and one band inherited from the mother. Second, digestion of this DNA with Hae III resulted in the visualization of just two bands at the D10S28 locus. Conclusion: The results confirm the notion that mutations at VNTR loci are not always the result of additions or deletions of tandem repeats, but that they can also involve single‐base substitutions either within or flanking VNTR loci that give rise to atypical DNA profiles and new alleles at the locus. single‐base changes within or flanking VNTR loci that give rise to a typical DNA profiles and new alleles at the locus. 1994 AABB",
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Single‐base substitutions give rise to a five‐banded DNA profile at the D10S28 locus. / Allen, Robert; Sanford‐Sharp, C. E.

In: Transfusion, Vol. 34, No. 5, 01.01.1994, p. 412-414.

Research output: Contribution to journalArticle

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