TY - JOUR
T1 - Simultaneous quantification of vinblastine and desacetylvinblastine concentrations in canine plasma and urine samples using LC-APCI-MS/MS
AU - Achanta, Satyanarayana
AU - Ngo, Minh
AU - Veitenheimer, Allison
AU - Maxwell, Lara K.
AU - Wagner, Jarrad R.
N1 - Funding Information:
This work was supported by the Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK, USA .
PY - 2013/1/15
Y1 - 2013/1/15
N2 - A highly sensitive and specific liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry (LC/APCI-MS/MS) method has been developed and validated for simultaneous quantification of vinblastine and its metabolite, desacetylvinblastine, in canine plasma and urine samples. Plasma and urine samples were processed by a solid phase extraction procedure. The optimal chromatographic behavior of these analytes was achieved on pentafluorophenyl (PFP) propyl analytical column (5μm, 50 × 2.1. mm) under isocratic elution of 0.75. mL/min with a mobile phase of 5. mM ammonium acetate and methanol. The samples were analyzed in positive ion, multiple reaction monitoring mode. The calibration curves were linear over 0.125-2. ng/mL (lower calibration curve); 2-100. ng/mL (higher calibration curve) and 0.125-5. ng/mL for vinblastine and desacetylvinblastine in plasma, and over 1-2000. ng/mL and 0.5-100. ng/mL for vinblastine and desacetylvinblastine in urine samples, respectively. The limits of quantitation of vinblastine and desacetylvinblastine were 0.125. ng/mL in both matrices. The intra and interday accuracy was above 89% and precision below 8.6% for both analytes in both matrices. The developed method was successfully applied to ongoing in vivo vinblastine pharmacokinetic studies in dogs.
AB - A highly sensitive and specific liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry (LC/APCI-MS/MS) method has been developed and validated for simultaneous quantification of vinblastine and its metabolite, desacetylvinblastine, in canine plasma and urine samples. Plasma and urine samples were processed by a solid phase extraction procedure. The optimal chromatographic behavior of these analytes was achieved on pentafluorophenyl (PFP) propyl analytical column (5μm, 50 × 2.1. mm) under isocratic elution of 0.75. mL/min with a mobile phase of 5. mM ammonium acetate and methanol. The samples were analyzed in positive ion, multiple reaction monitoring mode. The calibration curves were linear over 0.125-2. ng/mL (lower calibration curve); 2-100. ng/mL (higher calibration curve) and 0.125-5. ng/mL for vinblastine and desacetylvinblastine in plasma, and over 1-2000. ng/mL and 0.5-100. ng/mL for vinblastine and desacetylvinblastine in urine samples, respectively. The limits of quantitation of vinblastine and desacetylvinblastine were 0.125. ng/mL in both matrices. The intra and interday accuracy was above 89% and precision below 8.6% for both analytes in both matrices. The developed method was successfully applied to ongoing in vivo vinblastine pharmacokinetic studies in dogs.
KW - Analysis
KW - Desacetylvinblastine
KW - Dog
KW - LC/MS/MS
KW - Vinblastine
KW - Vinorelbine
UR - http://www.scopus.com/inward/record.url?scp=84872461453&partnerID=8YFLogxK
U2 - 10.1016/j.jchromb.2012.11.012
DO - 10.1016/j.jchromb.2012.11.012
M3 - Article
C2 - 23314352
AN - SCOPUS:84872461453
SN - 1570-0232
VL - 913-914
SP - 147
EP - 154
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
ER -