Simultaneous quantification of vinblastine and desacetylvinblastine concentrations in canine plasma and urine samples using LC-APCI-MS/MS

Satyanarayana Achanta, Minh Ngo, Allison Veitenheimer, Lara K. Maxwell, Jarrad R. Wagner

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

A highly sensitive and specific liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry (LC/APCI-MS/MS) method has been developed and validated for simultaneous quantification of vinblastine and its metabolite, desacetylvinblastine, in canine plasma and urine samples. Plasma and urine samples were processed by a solid phase extraction procedure. The optimal chromatographic behavior of these analytes was achieved on pentafluorophenyl (PFP) propyl analytical column (5μm, 50 × 2.1. mm) under isocratic elution of 0.75. mL/min with a mobile phase of 5. mM ammonium acetate and methanol. The samples were analyzed in positive ion, multiple reaction monitoring mode. The calibration curves were linear over 0.125-2. ng/mL (lower calibration curve); 2-100. ng/mL (higher calibration curve) and 0.125-5. ng/mL for vinblastine and desacetylvinblastine in plasma, and over 1-2000. ng/mL and 0.5-100. ng/mL for vinblastine and desacetylvinblastine in urine samples, respectively. The limits of quantitation of vinblastine and desacetylvinblastine were 0.125. ng/mL in both matrices. The intra and interday accuracy was above 89% and precision below 8.6% for both analytes in both matrices. The developed method was successfully applied to ongoing in vivo vinblastine pharmacokinetic studies in dogs.

Original languageEnglish
Pages (from-to)147-154
Number of pages8
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume913-914
DOIs
StatePublished - 15 Jan 2013

Fingerprint

Vinblastine
Canidae
Urine
Plasmas
Calibration
Atmospheric Pressure
Pharmacokinetics
Solid Phase Extraction
Liquid chromatography
Metabolites
Liquid Chromatography
Atmospheric pressure
Ionization
Mass spectrometry
Methanol
Mass Spectrometry
Positive ions
Dogs
Ions
Monitoring

Keywords

  • Analysis
  • Desacetylvinblastine
  • Dog
  • LC/MS/MS
  • Vinblastine
  • Vinorelbine

Cite this

@article{495e768dde554af293150bee48656201,
title = "Simultaneous quantification of vinblastine and desacetylvinblastine concentrations in canine plasma and urine samples using LC-APCI-MS/MS",
abstract = "A highly sensitive and specific liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry (LC/APCI-MS/MS) method has been developed and validated for simultaneous quantification of vinblastine and its metabolite, desacetylvinblastine, in canine plasma and urine samples. Plasma and urine samples were processed by a solid phase extraction procedure. The optimal chromatographic behavior of these analytes was achieved on pentafluorophenyl (PFP) propyl analytical column (5μm, 50 × 2.1. mm) under isocratic elution of 0.75. mL/min with a mobile phase of 5. mM ammonium acetate and methanol. The samples were analyzed in positive ion, multiple reaction monitoring mode. The calibration curves were linear over 0.125-2. ng/mL (lower calibration curve); 2-100. ng/mL (higher calibration curve) and 0.125-5. ng/mL for vinblastine and desacetylvinblastine in plasma, and over 1-2000. ng/mL and 0.5-100. ng/mL for vinblastine and desacetylvinblastine in urine samples, respectively. The limits of quantitation of vinblastine and desacetylvinblastine were 0.125. ng/mL in both matrices. The intra and interday accuracy was above 89{\%} and precision below 8.6{\%} for both analytes in both matrices. The developed method was successfully applied to ongoing in vivo vinblastine pharmacokinetic studies in dogs.",
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Simultaneous quantification of vinblastine and desacetylvinblastine concentrations in canine plasma and urine samples using LC-APCI-MS/MS. / Achanta, Satyanarayana; Ngo, Minh; Veitenheimer, Allison; Maxwell, Lara K.; Wagner, Jarrad R.

In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 913-914, 15.01.2013, p. 147-154.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Simultaneous quantification of vinblastine and desacetylvinblastine concentrations in canine plasma and urine samples using LC-APCI-MS/MS

AU - Achanta, Satyanarayana

AU - Ngo, Minh

AU - Veitenheimer, Allison

AU - Maxwell, Lara K.

AU - Wagner, Jarrad R.

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N2 - A highly sensitive and specific liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry (LC/APCI-MS/MS) method has been developed and validated for simultaneous quantification of vinblastine and its metabolite, desacetylvinblastine, in canine plasma and urine samples. Plasma and urine samples were processed by a solid phase extraction procedure. The optimal chromatographic behavior of these analytes was achieved on pentafluorophenyl (PFP) propyl analytical column (5μm, 50 × 2.1. mm) under isocratic elution of 0.75. mL/min with a mobile phase of 5. mM ammonium acetate and methanol. The samples were analyzed in positive ion, multiple reaction monitoring mode. The calibration curves were linear over 0.125-2. ng/mL (lower calibration curve); 2-100. ng/mL (higher calibration curve) and 0.125-5. ng/mL for vinblastine and desacetylvinblastine in plasma, and over 1-2000. ng/mL and 0.5-100. ng/mL for vinblastine and desacetylvinblastine in urine samples, respectively. The limits of quantitation of vinblastine and desacetylvinblastine were 0.125. ng/mL in both matrices. The intra and interday accuracy was above 89% and precision below 8.6% for both analytes in both matrices. The developed method was successfully applied to ongoing in vivo vinblastine pharmacokinetic studies in dogs.

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KW - Analysis

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KW - LC/MS/MS

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KW - Vinorelbine

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