Simultaneous determination of the enantiomers of esmolol and its acid metabolite in human plasma by reversed phase liquid chromatography with solid-phase extraction

Yi Hong Tang; Ying He; Tong Wei Yao; Su Zeng

doi:10.1016/j.jchromb.2004.03.007

Simultaneous determination of the enantiomers of esmolol and its acid metabolite in human plasma by reversed phase liquid chromatography with solid-phase extraction

Yi Hong Tang, Ying He, Tong Wei Yao, Su Zeng

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

A stereoselective RP-high performance liquid chromatography (HPLC) assay to determine simultaneously the enantiomers of esmolol and its acid metabolite in human plasma was developed. The method involved a solid-phase extraction and a reversed-phase chromatographic separation with UV detection (λ=224 nm) after chiral derivatization. 2,3,4,6-tetra-O-acetyl-β-D-glucopyranosyl isothiocyanate (GITC) was employed as a pre-column chiral derivatization reagent. The assay was linear from 0.09 to 8.0 μg/ml for each enantiomer of esmolol and 0.07-8.0 μg/ml for each enantiomer of the acid metabolite. The absolute recoveries for all enantiomers were >73%. The intra- and inter-day variations were <15%. The validated method was applied to quantify the enantiomers of esmolol and its metabolite in human plasma for hydrolysis studies.

Original language	English
Pages (from-to)	249-254
Number of pages	6
Journal	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume	805
Issue number	2
DOIs	https://doi.org/10.1016/j.jchromb.2004.03.007
State	Published - 15 Jun 2004
Externally published	Yes

Keywords

Enantiomer separation
Esmolol

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10.1016/j.jchromb.2004.03.007

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Tang, Y. H., He, Y., Yao, T. W., & Zeng, S. (2004). Simultaneous determination of the enantiomers of esmolol and its acid metabolite in human plasma by reversed phase liquid chromatography with solid-phase extraction. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 805(2), 249-254. https://doi.org/10.1016/j.jchromb.2004.03.007

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title = "Simultaneous determination of the enantiomers of esmolol and its acid metabolite in human plasma by reversed phase liquid chromatography with solid-phase extraction",

abstract = "A stereoselective RP-high performance liquid chromatography (HPLC) assay to determine simultaneously the enantiomers of esmolol and its acid metabolite in human plasma was developed. The method involved a solid-phase extraction and a reversed-phase chromatographic separation with UV detection (λ=224 nm) after chiral derivatization. 2,3,4,6-tetra-O-acetyl-β-D-glucopyranosyl isothiocyanate (GITC) was employed as a pre-column chiral derivatization reagent. The assay was linear from 0.09 to 8.0 μg/ml for each enantiomer of esmolol and 0.07-8.0 μg/ml for each enantiomer of the acid metabolite. The absolute recoveries for all enantiomers were >73%. The intra- and inter-day variations were <15%. The validated method was applied to quantify the enantiomers of esmolol and its metabolite in human plasma for hydrolysis studies.",

keywords = "Enantiomer separation, Esmolol",

author = "Tang, {Yi Hong} and Ying He and Yao, {Tong Wei} and Su Zeng",

note = "Funding Information: Project was supported by Natural Science Found of China (30225047, 39770868), and Zhejiang Provincial Natural Science Foundation of China (#RC97016). ",

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Tang, YH, He, Y, Yao, TW & Zeng, S 2004, 'Simultaneous determination of the enantiomers of esmolol and its acid metabolite in human plasma by reversed phase liquid chromatography with solid-phase extraction', Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, vol. 805, no. 2, pp. 249-254. https://doi.org/10.1016/j.jchromb.2004.03.007

Simultaneous determination of the enantiomers of esmolol and its acid metabolite in human plasma by reversed phase liquid chromatography with solid-phase extraction. / Tang, Yi Hong; He, Ying; Yao, Tong Wei et al.
In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 805, No. 2, 15.06.2004, p. 249-254.

Research output: Contribution to journal › Article › peer-review

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T1 - Simultaneous determination of the enantiomers of esmolol and its acid metabolite in human plasma by reversed phase liquid chromatography with solid-phase extraction

AU - Tang, Yi Hong

AU - He, Ying

AU - Yao, Tong Wei

AU - Zeng, Su

N1 - Funding Information: Project was supported by Natural Science Found of China (30225047, 39770868), and Zhejiang Provincial Natural Science Foundation of China (#RC97016).

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N2 - A stereoselective RP-high performance liquid chromatography (HPLC) assay to determine simultaneously the enantiomers of esmolol and its acid metabolite in human plasma was developed. The method involved a solid-phase extraction and a reversed-phase chromatographic separation with UV detection (λ=224 nm) after chiral derivatization. 2,3,4,6-tetra-O-acetyl-β-D-glucopyranosyl isothiocyanate (GITC) was employed as a pre-column chiral derivatization reagent. The assay was linear from 0.09 to 8.0 μg/ml for each enantiomer of esmolol and 0.07-8.0 μg/ml for each enantiomer of the acid metabolite. The absolute recoveries for all enantiomers were >73%. The intra- and inter-day variations were <15%. The validated method was applied to quantify the enantiomers of esmolol and its metabolite in human plasma for hydrolysis studies.

AB - A stereoselective RP-high performance liquid chromatography (HPLC) assay to determine simultaneously the enantiomers of esmolol and its acid metabolite in human plasma was developed. The method involved a solid-phase extraction and a reversed-phase chromatographic separation with UV detection (λ=224 nm) after chiral derivatization. 2,3,4,6-tetra-O-acetyl-β-D-glucopyranosyl isothiocyanate (GITC) was employed as a pre-column chiral derivatization reagent. The assay was linear from 0.09 to 8.0 μg/ml for each enantiomer of esmolol and 0.07-8.0 μg/ml for each enantiomer of the acid metabolite. The absolute recoveries for all enantiomers were >73%. The intra- and inter-day variations were <15%. The validated method was applied to quantify the enantiomers of esmolol and its metabolite in human plasma for hydrolysis studies.

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Simultaneous determination of the enantiomers of esmolol and its acid metabolite in human plasma by reversed phase liquid chromatography with solid-phase extraction

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