Simultaneous determination of the enantiomers of esmolol and its acid metabolite in human plasma by reversed phase liquid chromatography with solid-phase extraction

Yi Hong Tang, Ying He, Tong Wei Yao, Su Zeng

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11 Scopus citations

Abstract

A stereoselective RP-high performance liquid chromatography (HPLC) assay to determine simultaneously the enantiomers of esmolol and its acid metabolite in human plasma was developed. The method involved a solid-phase extraction and a reversed-phase chromatographic separation with UV detection (λ=224 nm) after chiral derivatization. 2,3,4,6-tetra-O-acetyl-β-D-glucopyranosyl isothiocyanate (GITC) was employed as a pre-column chiral derivatization reagent. The assay was linear from 0.09 to 8.0 μg/ml for each enantiomer of esmolol and 0.07-8.0 μg/ml for each enantiomer of the acid metabolite. The absolute recoveries for all enantiomers were >73%. The intra- and inter-day variations were <15%. The validated method was applied to quantify the enantiomers of esmolol and its metabolite in human plasma for hydrolysis studies.

Original languageEnglish
Pages (from-to)249-254
Number of pages6
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume805
Issue number2
DOIs
StatePublished - 15 Jun 2004
Externally publishedYes

Keywords

  • Enantiomer separation
  • Esmolol

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