Resolution of a serum sample mix-up through the use of short tandem repeat DNA typing

Robert Allen, Jane K. Pritchard

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

BACKGROUND: A sample mix-up occurred in a tissue procurement laboratory in which aliquots of serum from two tissue donors were accidentally mislabeled. The clues to the apparent mixup involved discrepant Hepatitus C test results. In an attempt to resolve the apparent mix up, DNA typing was performed using serum samples as a possible source of genomic DNA. STUDY DESIGN AMD METHODS: Two hundred microliter aliquots of two reference sera and aliquots prepared from them were subjected to DNA extraction. PCR amplification of 9 STR loci was performed on the extracts and amplicons were analyzed by capillary electrophoresis. RESULTS: About 1 μg/ml of DNA was recovered from all serum samples and was of sufficient quality to direct the amplification of most, if not all STR loci allowing the mislabeled specimens to be traced to the proper tissue donor. CONCLUSIONS: Serum is a useful source of genomic DNA for STR analysis in situations in which such samples are the only source of DNA for testing. Interestingly, one of the tissue donors on life support and repeatedly receiving blood products, exhibited a mixed DNA profile indicative of the presence of DNA from multiple individuals in the bloodstream.

Original languageEnglish
Pages (from-to)1750-1754
Number of pages5
JournalTransfusion
Volume44
Issue number12
DOIs
StatePublished - 1 Dec 2004

Fingerprint

DNA Fingerprinting
Microsatellite Repeats
DNA
Serum
Tissue Donors
Tissue and Organ Procurement
Capillary Electrophoresis
Polymerase Chain Reaction

Cite this

@article{695d99f3006e494f9e60a36748d816e7,
title = "Resolution of a serum sample mix-up through the use of short tandem repeat DNA typing",
abstract = "BACKGROUND: A sample mix-up occurred in a tissue procurement laboratory in which aliquots of serum from two tissue donors were accidentally mislabeled. The clues to the apparent mixup involved discrepant Hepatitus C test results. In an attempt to resolve the apparent mix up, DNA typing was performed using serum samples as a possible source of genomic DNA. STUDY DESIGN AMD METHODS: Two hundred microliter aliquots of two reference sera and aliquots prepared from them were subjected to DNA extraction. PCR amplification of 9 STR loci was performed on the extracts and amplicons were analyzed by capillary electrophoresis. RESULTS: About 1 μg/ml of DNA was recovered from all serum samples and was of sufficient quality to direct the amplification of most, if not all STR loci allowing the mislabeled specimens to be traced to the proper tissue donor. CONCLUSIONS: Serum is a useful source of genomic DNA for STR analysis in situations in which such samples are the only source of DNA for testing. Interestingly, one of the tissue donors on life support and repeatedly receiving blood products, exhibited a mixed DNA profile indicative of the presence of DNA from multiple individuals in the bloodstream.",
author = "Robert Allen and Pritchard, {Jane K.}",
year = "2004",
month = "12",
day = "1",
doi = "10.1111/j.0041-1132.2004.04212.x",
language = "English",
volume = "44",
pages = "1750--1754",
journal = "Transfusion",
issn = "0041-1132",
publisher = "Wiley-Blackwell Publishing Ltd",
number = "12",

}

Resolution of a serum sample mix-up through the use of short tandem repeat DNA typing. / Allen, Robert; Pritchard, Jane K.

In: Transfusion, Vol. 44, No. 12, 01.12.2004, p. 1750-1754.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Resolution of a serum sample mix-up through the use of short tandem repeat DNA typing

AU - Allen, Robert

AU - Pritchard, Jane K.

PY - 2004/12/1

Y1 - 2004/12/1

N2 - BACKGROUND: A sample mix-up occurred in a tissue procurement laboratory in which aliquots of serum from two tissue donors were accidentally mislabeled. The clues to the apparent mixup involved discrepant Hepatitus C test results. In an attempt to resolve the apparent mix up, DNA typing was performed using serum samples as a possible source of genomic DNA. STUDY DESIGN AMD METHODS: Two hundred microliter aliquots of two reference sera and aliquots prepared from them were subjected to DNA extraction. PCR amplification of 9 STR loci was performed on the extracts and amplicons were analyzed by capillary electrophoresis. RESULTS: About 1 μg/ml of DNA was recovered from all serum samples and was of sufficient quality to direct the amplification of most, if not all STR loci allowing the mislabeled specimens to be traced to the proper tissue donor. CONCLUSIONS: Serum is a useful source of genomic DNA for STR analysis in situations in which such samples are the only source of DNA for testing. Interestingly, one of the tissue donors on life support and repeatedly receiving blood products, exhibited a mixed DNA profile indicative of the presence of DNA from multiple individuals in the bloodstream.

AB - BACKGROUND: A sample mix-up occurred in a tissue procurement laboratory in which aliquots of serum from two tissue donors were accidentally mislabeled. The clues to the apparent mixup involved discrepant Hepatitus C test results. In an attempt to resolve the apparent mix up, DNA typing was performed using serum samples as a possible source of genomic DNA. STUDY DESIGN AMD METHODS: Two hundred microliter aliquots of two reference sera and aliquots prepared from them were subjected to DNA extraction. PCR amplification of 9 STR loci was performed on the extracts and amplicons were analyzed by capillary electrophoresis. RESULTS: About 1 μg/ml of DNA was recovered from all serum samples and was of sufficient quality to direct the amplification of most, if not all STR loci allowing the mislabeled specimens to be traced to the proper tissue donor. CONCLUSIONS: Serum is a useful source of genomic DNA for STR analysis in situations in which such samples are the only source of DNA for testing. Interestingly, one of the tissue donors on life support and repeatedly receiving blood products, exhibited a mixed DNA profile indicative of the presence of DNA from multiple individuals in the bloodstream.

UR - http://www.scopus.com/inward/record.url?scp=10444231954&partnerID=8YFLogxK

U2 - 10.1111/j.0041-1132.2004.04212.x

DO - 10.1111/j.0041-1132.2004.04212.x

M3 - Article

C2 - 15584990

AN - SCOPUS:10444231954

VL - 44

SP - 1750

EP - 1754

JO - Transfusion

JF - Transfusion

SN - 0041-1132

IS - 12

ER -