Relationship of IL-19 to the metastatic potential of a lung tumor

Joshua Rothenberg, Russell Zhuravsky, Marlow B. Hernandez, Natasha Bray, Tony Weaver

    Research output: Contribution to journalArticle

    Abstract

    Interleukin 19 (IL-19), an anti-inflammatory and immunosuppressant cytokine, has been shown to affect the function and viability of lung fibroblasts, and has been implicated in lung cancer metastasis. 1-4 The purpose of this research was to compare gene expression profiles of IL-19 genes (through the collection of RNA isolates) between two lines of mice, an original tumor line (DA-3/TM), and a surgically resected metastatic tumor line (DA-3/TM-Mets). A battery of laboratory procedures were used to study metastasis of cancer from a primary tumor cell line, DA-3/TM, and from a metastatic site, DA-3/TM-Mets. Metastatic lesions in animal models were isolated, cultured, and analyzed in order to observe the changes between the primary and metastatic line. Both PCR and then Western Blots were performed to verify the initial findings from the metastatic lesions of mice. It was found that DA-3/TM-Mets, had more nodule metastasis throughout the lungs of BALB/c mice compared to that of DA-3/TM. A gene array performed showed that the key difference was IL-19, which was 30X higher in DA-3/TM-Mets than in DA-3/TM. This suggests a significant difference at the gene level. However, it is important to develop a new model to look at these tumor cell lines at the protein level in order to observe if this difference holds true. The data acquired shows promising results. Higher concentrations of IL-19 in the DA-3/ TM- Mets line may lead to the conclusion that IL-19 is exhibiting immunosuppressive activity, which does not allow the BALB/c mice's immune system to fight off a tumor, resulting in their death. Therefore, there appears to be a direct correlation between the metastasis of cancer and the protein IL-19's immunosuppressive effects.

    Original languageEnglish
    JournalInternet Journal of Oncology
    Volume7
    Issue number1
    StatePublished - 8 Jul 2010

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    Interleukins
    Lung
    Immunosuppressive Agents
    Neoplasm Metastasis
    Neoplasms
    Tumor Cell Line
    Genes
    Transcriptome
    Immune System
    Lung Neoplasms
    Proteins
    Anti-Inflammatory Agents
    Animal Models
    Fibroblasts
    Western Blotting
    RNA
    Cytokines
    Polymerase Chain Reaction
    Research

    Cite this

    Rothenberg, J., Zhuravsky, R., Hernandez, M. B., Bray, N., & Weaver, T. (2010). Relationship of IL-19 to the metastatic potential of a lung tumor. Internet Journal of Oncology, 7(1).
    Rothenberg, Joshua ; Zhuravsky, Russell ; Hernandez, Marlow B. ; Bray, Natasha ; Weaver, Tony. / Relationship of IL-19 to the metastatic potential of a lung tumor. In: Internet Journal of Oncology. 2010 ; Vol. 7, No. 1.
    @article{73c77d357dc84eb19dc941a6ada89f01,
    title = "Relationship of IL-19 to the metastatic potential of a lung tumor",
    abstract = "Interleukin 19 (IL-19), an anti-inflammatory and immunosuppressant cytokine, has been shown to affect the function and viability of lung fibroblasts, and has been implicated in lung cancer metastasis. 1-4 The purpose of this research was to compare gene expression profiles of IL-19 genes (through the collection of RNA isolates) between two lines of mice, an original tumor line (DA-3/TM), and a surgically resected metastatic tumor line (DA-3/TM-Mets). A battery of laboratory procedures were used to study metastasis of cancer from a primary tumor cell line, DA-3/TM, and from a metastatic site, DA-3/TM-Mets. Metastatic lesions in animal models were isolated, cultured, and analyzed in order to observe the changes between the primary and metastatic line. Both PCR and then Western Blots were performed to verify the initial findings from the metastatic lesions of mice. It was found that DA-3/TM-Mets, had more nodule metastasis throughout the lungs of BALB/c mice compared to that of DA-3/TM. A gene array performed showed that the key difference was IL-19, which was 30X higher in DA-3/TM-Mets than in DA-3/TM. This suggests a significant difference at the gene level. However, it is important to develop a new model to look at these tumor cell lines at the protein level in order to observe if this difference holds true. The data acquired shows promising results. Higher concentrations of IL-19 in the DA-3/ TM- Mets line may lead to the conclusion that IL-19 is exhibiting immunosuppressive activity, which does not allow the BALB/c mice's immune system to fight off a tumor, resulting in their death. Therefore, there appears to be a direct correlation between the metastasis of cancer and the protein IL-19's immunosuppressive effects.",
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    Rothenberg, J, Zhuravsky, R, Hernandez, MB, Bray, N & Weaver, T 2010, 'Relationship of IL-19 to the metastatic potential of a lung tumor', Internet Journal of Oncology, vol. 7, no. 1.

    Relationship of IL-19 to the metastatic potential of a lung tumor. / Rothenberg, Joshua; Zhuravsky, Russell; Hernandez, Marlow B.; Bray, Natasha; Weaver, Tony.

    In: Internet Journal of Oncology, Vol. 7, No. 1, 08.07.2010.

    Research output: Contribution to journalArticle

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    AU - Zhuravsky, Russell

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    AU - Weaver, Tony

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    Rothenberg J, Zhuravsky R, Hernandez MB, Bray N, Weaver T. Relationship of IL-19 to the metastatic potential of a lung tumor. Internet Journal of Oncology. 2010 Jul 8;7(1).