Regional differences in serotonin content in the nucleus of the solitary tract of male rats after hypovolemia produced by polyethylene glycol

J. Thomas Curtis; Michael B. Anderson; Kathleen S. Curtis

doi:10.1007/s12576-012-0229-4

Regional differences in serotonin content in the nucleus of the solitary tract of male rats after hypovolemia produced by polyethylene glycol

J. Thomas Curtis
, Michael B. Anderson
, Kathleen S. Curtis

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Serotonin (5-HT) has been implicated in centrally mediated compensatory responses to volume loss in rats. Accordingly, we hypothesized that slowly developing, non-hypotensive hypovolemia increases serotonin in the hindbrain nucleus of the solitary tract (NTS). We produced volume loss in adult male rats by administering hyperoncotic polyethylene glycol (PEG) and then assessed 5-HT levels in the NTS using measurements of tissue 5-HT content or 5-HT immunohistochemistry. The results show selective increases of 5-HT in the caudal NTS after PEG treatment, but no change in the primary 5-HT metabolite, 5-HIAA. Moreover, the intensity of 5-HT immunolabeled fibers in the caudal NTS was increased after PEG treatment. These findings suggest that, after PEG-induced hypovolemia, 5-HT accumulates in neural elements in the caudal NTS. We propose that this accumulation is attributable to an initial release of 5-HT that then acts at presynaptic autoreceptors to inhibit subsequent 5-HT release.

Original language	English
Pages (from-to)	39-46
Number of pages	8
Journal	Journal of Physiological Sciences
Volume	63
Issue number	1
DOIs	https://doi.org/10.1007/s12576-012-0229-4
State	Published - Jan 2013

Keywords

5-HIAA
Baroreflex pathway
Hemorrhage
Norepinephrine

Access to Document

10.1007/s12576-012-0229-4

Cite this

@article{59149102bd07496a90b8fe5e849a4dd7,

title = "Regional differences in serotonin content in the nucleus of the solitary tract of male rats after hypovolemia produced by polyethylene glycol",

abstract = "Serotonin (5-HT) has been implicated in centrally mediated compensatory responses to volume loss in rats. Accordingly, we hypothesized that slowly developing, non-hypotensive hypovolemia increases serotonin in the hindbrain nucleus of the solitary tract (NTS). We produced volume loss in adult male rats by administering hyperoncotic polyethylene glycol (PEG) and then assessed 5-HT levels in the NTS using measurements of tissue 5-HT content or 5-HT immunohistochemistry. The results show selective increases of 5-HT in the caudal NTS after PEG treatment, but no change in the primary 5-HT metabolite, 5-HIAA. Moreover, the intensity of 5-HT immunolabeled fibers in the caudal NTS was increased after PEG treatment. These findings suggest that, after PEG-induced hypovolemia, 5-HT accumulates in neural elements in the caudal NTS. We propose that this accumulation is attributable to an initial release of 5-HT that then acts at presynaptic autoreceptors to inhibit subsequent 5-HT release.",

keywords = "5-HIAA, Baroreflex pathway, Hemorrhage, Norepinephrine",

author = "Curtis, \{J. Thomas\} and Anderson, \{Michael B.\} and Curtis, \{Kathleen S.\}",

note = "Funding Information: Acknowledgments Portions of these data were presented at the annual meeting of the Society for Neuroscience (Washington, DC; 2005). Grants from the National Institute on Deafness and Communication Disorders DC-06360 (K.S.C.), the National Institute for Child Health and Development HD-48462 (J.T.C.), the Oklahoma Center for the Advancement of Science and Technology Health Research Program HR 09-123 (K.S.C.), and Oklahoma EPSCoR (M.B.A.) supported this research. The authors thank Drs. Rob Con-treras, Zuoxin Wang, and Marc Freeman for their generosity with equipment and expertise and Ms. De{\textquoteright}Nise McKee for technical advice and assistance.",

year = "2013",

month = jan,

doi = "10.1007/s12576-012-0229-4",

language = "English",

volume = "63",

pages = "39--46",

journal = "Journal of Physiological Sciences",

issn = "1880-6546",

publisher = "Elsevier Inc.",

number = "1",

}

TY - JOUR

T1 - Regional differences in serotonin content in the nucleus of the solitary tract of male rats after hypovolemia produced by polyethylene glycol

AU - Curtis, J. Thomas

AU - Anderson, Michael B.

AU - Curtis, Kathleen S.

N1 - Funding Information: Acknowledgments Portions of these data were presented at the annual meeting of the Society for Neuroscience (Washington, DC; 2005). Grants from the National Institute on Deafness and Communication Disorders DC-06360 (K.S.C.), the National Institute for Child Health and Development HD-48462 (J.T.C.), the Oklahoma Center for the Advancement of Science and Technology Health Research Program HR 09-123 (K.S.C.), and Oklahoma EPSCoR (M.B.A.) supported this research. The authors thank Drs. Rob Con-treras, Zuoxin Wang, and Marc Freeman for their generosity with equipment and expertise and Ms. De’Nise McKee for technical advice and assistance.

PY - 2013/1

Y1 - 2013/1

N2 - Serotonin (5-HT) has been implicated in centrally mediated compensatory responses to volume loss in rats. Accordingly, we hypothesized that slowly developing, non-hypotensive hypovolemia increases serotonin in the hindbrain nucleus of the solitary tract (NTS). We produced volume loss in adult male rats by administering hyperoncotic polyethylene glycol (PEG) and then assessed 5-HT levels in the NTS using measurements of tissue 5-HT content or 5-HT immunohistochemistry. The results show selective increases of 5-HT in the caudal NTS after PEG treatment, but no change in the primary 5-HT metabolite, 5-HIAA. Moreover, the intensity of 5-HT immunolabeled fibers in the caudal NTS was increased after PEG treatment. These findings suggest that, after PEG-induced hypovolemia, 5-HT accumulates in neural elements in the caudal NTS. We propose that this accumulation is attributable to an initial release of 5-HT that then acts at presynaptic autoreceptors to inhibit subsequent 5-HT release.

AB - Serotonin (5-HT) has been implicated in centrally mediated compensatory responses to volume loss in rats. Accordingly, we hypothesized that slowly developing, non-hypotensive hypovolemia increases serotonin in the hindbrain nucleus of the solitary tract (NTS). We produced volume loss in adult male rats by administering hyperoncotic polyethylene glycol (PEG) and then assessed 5-HT levels in the NTS using measurements of tissue 5-HT content or 5-HT immunohistochemistry. The results show selective increases of 5-HT in the caudal NTS after PEG treatment, but no change in the primary 5-HT metabolite, 5-HIAA. Moreover, the intensity of 5-HT immunolabeled fibers in the caudal NTS was increased after PEG treatment. These findings suggest that, after PEG-induced hypovolemia, 5-HT accumulates in neural elements in the caudal NTS. We propose that this accumulation is attributable to an initial release of 5-HT that then acts at presynaptic autoreceptors to inhibit subsequent 5-HT release.

KW - 5-HIAA

KW - Baroreflex pathway

KW - Hemorrhage

KW - Norepinephrine

UR - https://www.scopus.com/pages/publications/84871666150

U2 - 10.1007/s12576-012-0229-4

DO - 10.1007/s12576-012-0229-4

M3 - Article

C2 - 22945371

AN - SCOPUS:84871666150

SN - 1880-6546

VL - 63

SP - 39

EP - 46

JO - Journal of Physiological Sciences

JF - Journal of Physiological Sciences

IS - 1

ER -

Regional differences in serotonin content in the nucleus of the solitary tract of male rats after hypovolemia produced by polyethylene glycol

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this