Regional differences in serotonin content in the nucleus of the solitary tract of male rats after hypovolemia produced by polyethylene glycol

J. Thomas Curtis; Michael B. Anderson; Kathleen S. Curtis

doi:10.1007/s12576-012-0229-4

Regional differences in serotonin content in the nucleus of the solitary tract of male rats after hypovolemia produced by polyethylene glycol

J. Thomas Curtis, Michael B. Anderson, Kathleen S. Curtis

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2 Scopus citations

Abstract

Serotonin (5-HT) has been implicated in centrally mediated compensatory responses to volume loss in rats. Accordingly, we hypothesized that slowly developing, non-hypotensive hypovolemia increases serotonin in the hindbrain nucleus of the solitary tract (NTS). We produced volume loss in adult male rats by administering hyperoncotic polyethylene glycol (PEG) and then assessed 5-HT levels in the NTS using measurements of tissue 5-HT content or 5-HT immunohistochemistry. The results show selective increases of 5-HT in the caudal NTS after PEG treatment, but no change in the primary 5-HT metabolite, 5-HIAA. Moreover, the intensity of 5-HT immunolabeled fibers in the caudal NTS was increased after PEG treatment. These findings suggest that, after PEG-induced hypovolemia, 5-HT accumulates in neural elements in the caudal NTS. We propose that this accumulation is attributable to an initial release of 5-HT that then acts at presynaptic autoreceptors to inhibit subsequent 5-HT release.

Original language	English
Pages (from-to)	39-46
Number of pages	8
Journal	Journal of Physiological Sciences
Volume	63
Issue number	1
DOIs	https://doi.org/10.1007/s12576-012-0229-4
State	Published - Jan 2013

Keywords

5-HIAA
Baroreflex pathway
Hemorrhage
Norepinephrine

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10.1007/s12576-012-0229-4

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title = "Regional differences in serotonin content in the nucleus of the solitary tract of male rats after hypovolemia produced by polyethylene glycol",

abstract = "Serotonin (5-HT) has been implicated in centrally mediated compensatory responses to volume loss in rats. Accordingly, we hypothesized that slowly developing, non-hypotensive hypovolemia increases serotonin in the hindbrain nucleus of the solitary tract (NTS). We produced volume loss in adult male rats by administering hyperoncotic polyethylene glycol (PEG) and then assessed 5-HT levels in the NTS using measurements of tissue 5-HT content or 5-HT immunohistochemistry. The results show selective increases of 5-HT in the caudal NTS after PEG treatment, but no change in the primary 5-HT metabolite, 5-HIAA. Moreover, the intensity of 5-HT immunolabeled fibers in the caudal NTS was increased after PEG treatment. These findings suggest that, after PEG-induced hypovolemia, 5-HT accumulates in neural elements in the caudal NTS. We propose that this accumulation is attributable to an initial release of 5-HT that then acts at presynaptic autoreceptors to inhibit subsequent 5-HT release.",

keywords = "5-HIAA, Baroreflex pathway, Hemorrhage, Norepinephrine",

author = "Curtis, {J. Thomas} and Anderson, {Michael B.} and Curtis, {Kathleen S.}",

note = "Funding Information: Acknowledgments Portions of these data were presented at the annual meeting of the Society for Neuroscience (Washington, DC; 2005). Grants from the National Institute on Deafness and Communication Disorders DC-06360 (K.S.C.), the National Institute for Child Health and Development HD-48462 (J.T.C.), the Oklahoma Center for the Advancement of Science and Technology Health Research Program HR 09-123 (K.S.C.), and Oklahoma EPSCoR (M.B.A.) supported this research. The authors thank Drs. Rob Con-treras, Zuoxin Wang, and Marc Freeman for their generosity with equipment and expertise and Ms. De{\textquoteright}Nise McKee for technical advice and assistance.",

year = "2013",

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doi = "10.1007/s12576-012-0229-4",

language = "English",

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T1 - Regional differences in serotonin content in the nucleus of the solitary tract of male rats after hypovolemia produced by polyethylene glycol

AU - Curtis, J. Thomas

AU - Anderson, Michael B.

AU - Curtis, Kathleen S.

N1 - Funding Information: Acknowledgments Portions of these data were presented at the annual meeting of the Society for Neuroscience (Washington, DC; 2005). Grants from the National Institute on Deafness and Communication Disorders DC-06360 (K.S.C.), the National Institute for Child Health and Development HD-48462 (J.T.C.), the Oklahoma Center for the Advancement of Science and Technology Health Research Program HR 09-123 (K.S.C.), and Oklahoma EPSCoR (M.B.A.) supported this research. The authors thank Drs. Rob Con-treras, Zuoxin Wang, and Marc Freeman for their generosity with equipment and expertise and Ms. De’Nise McKee for technical advice and assistance.

PY - 2013/1

Y1 - 2013/1

N2 - Serotonin (5-HT) has been implicated in centrally mediated compensatory responses to volume loss in rats. Accordingly, we hypothesized that slowly developing, non-hypotensive hypovolemia increases serotonin in the hindbrain nucleus of the solitary tract (NTS). We produced volume loss in adult male rats by administering hyperoncotic polyethylene glycol (PEG) and then assessed 5-HT levels in the NTS using measurements of tissue 5-HT content or 5-HT immunohistochemistry. The results show selective increases of 5-HT in the caudal NTS after PEG treatment, but no change in the primary 5-HT metabolite, 5-HIAA. Moreover, the intensity of 5-HT immunolabeled fibers in the caudal NTS was increased after PEG treatment. These findings suggest that, after PEG-induced hypovolemia, 5-HT accumulates in neural elements in the caudal NTS. We propose that this accumulation is attributable to an initial release of 5-HT that then acts at presynaptic autoreceptors to inhibit subsequent 5-HT release.

AB - Serotonin (5-HT) has been implicated in centrally mediated compensatory responses to volume loss in rats. Accordingly, we hypothesized that slowly developing, non-hypotensive hypovolemia increases serotonin in the hindbrain nucleus of the solitary tract (NTS). We produced volume loss in adult male rats by administering hyperoncotic polyethylene glycol (PEG) and then assessed 5-HT levels in the NTS using measurements of tissue 5-HT content or 5-HT immunohistochemistry. The results show selective increases of 5-HT in the caudal NTS after PEG treatment, but no change in the primary 5-HT metabolite, 5-HIAA. Moreover, the intensity of 5-HT immunolabeled fibers in the caudal NTS was increased after PEG treatment. These findings suggest that, after PEG-induced hypovolemia, 5-HT accumulates in neural elements in the caudal NTS. We propose that this accumulation is attributable to an initial release of 5-HT that then acts at presynaptic autoreceptors to inhibit subsequent 5-HT release.

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KW - Baroreflex pathway

KW - Hemorrhage

KW - Norepinephrine

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Regional differences in serotonin content in the nucleus of the solitary tract of male rats after hypovolemia produced by polyethylene glycol

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