Regional differences in estradiol effects on numbers of HSD2-containing neurons in the nucleus of the solitary tract of rats

Liming Fan, Courtney E. Smith, Kathleen Curtis

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Estrogens affect body fluid balance, including sodium ingestion. Recent findings of a population of neurons in the hindbrain nucleus of the solitary tract (NTS) of rats that are activated during sodium need suggest a possible central substrate for this effect of estrogens. We used immunohistochemistry to label neurons in the NTS that express 11-β-hydroxysteroid dehydrogenase type 2 (HSD2), an enzyme that promotes aldosterone binding, in male rats, and in ovariectomized (OVX) rats given estradiol benzoate (EB) or oil vehicle (OIL). During baseline conditions, the number of HSD2 immunoreactive neurons in the NTS immediately rostral to the area postrema was greater in EB-treated OVX rats compared to those in OIL-treated OVX and male rats. A small number of HSD2 immunoreactive neurons was also labeled for dopamine-β-hydroxylase (DBH), an enzyme involved in norepinephrine biosynthesis. Double-labeled neurons in the NTS were located primarily in the more lateral portion of the HSD2 population, at the level of the area postrema in all three groups, with no sex or estrogen-mediated differences in the number of double-labeled neurons. These results suggest that two subpopulations of HSD2 neurons are present in the NTS. One subpopulation, which does not colocalize with DBH and is increased during conditions of elevated estradiol, may contribute to the effects of estrogens on sodium ingestion. The role of the other, smaller subpopulation, which colocalizes with DBH and is not affected by estradiol, remains to be determined, but one possibility is that these latter neurons are part of a larger network of catecholaminergic input to neuroendocrine neurons in the hypothalamus.

Original languageEnglish
Pages (from-to)89-101
Number of pages13
JournalBrain Research
Volume1358
DOIs
StatePublished - 28 Oct 2010

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11-beta-Hydroxysteroid Dehydrogenases
Solitary Nucleus
Estradiol
Neurons
Estrogens
Area Postrema
Sodium
Eating
Rhombencephalon
Water-Electrolyte Balance
Body Fluids
Enzymes
Mixed Function Oxygenases
Aldosterone
Population
Hypothalamus
Dopamine
Norepinephrine
Oils
Immunohistochemistry

Keywords

  • Dopamine-β-hydroxylase
  • Ovariectomy
  • Salt intake
  • Sex difference

Cite this

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abstract = "Estrogens affect body fluid balance, including sodium ingestion. Recent findings of a population of neurons in the hindbrain nucleus of the solitary tract (NTS) of rats that are activated during sodium need suggest a possible central substrate for this effect of estrogens. We used immunohistochemistry to label neurons in the NTS that express 11-β-hydroxysteroid dehydrogenase type 2 (HSD2), an enzyme that promotes aldosterone binding, in male rats, and in ovariectomized (OVX) rats given estradiol benzoate (EB) or oil vehicle (OIL). During baseline conditions, the number of HSD2 immunoreactive neurons in the NTS immediately rostral to the area postrema was greater in EB-treated OVX rats compared to those in OIL-treated OVX and male rats. A small number of HSD2 immunoreactive neurons was also labeled for dopamine-β-hydroxylase (DBH), an enzyme involved in norepinephrine biosynthesis. Double-labeled neurons in the NTS were located primarily in the more lateral portion of the HSD2 population, at the level of the area postrema in all three groups, with no sex or estrogen-mediated differences in the number of double-labeled neurons. These results suggest that two subpopulations of HSD2 neurons are present in the NTS. One subpopulation, which does not colocalize with DBH and is increased during conditions of elevated estradiol, may contribute to the effects of estrogens on sodium ingestion. The role of the other, smaller subpopulation, which colocalizes with DBH and is not affected by estradiol, remains to be determined, but one possibility is that these latter neurons are part of a larger network of catecholaminergic input to neuroendocrine neurons in the hypothalamus.",
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Regional differences in estradiol effects on numbers of HSD2-containing neurons in the nucleus of the solitary tract of rats. / Fan, Liming; Smith, Courtney E.; Curtis, Kathleen.

In: Brain Research, Vol. 1358, 28.10.2010, p. 89-101.

Research output: Contribution to journalArticle

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AU - Fan, Liming

AU - Smith, Courtney E.

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