Quantitative amino acid analysis using a Beckman system gold HPLC 126AA analyzer

Yong Qu, Robert H. Slocum, Jun Fu, Wendy E. Rasmussen, Heather D. Rector, James B. Miller, James G. Coldwell

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Abstract

Background: The Beckman 6300/7300 analyzer, which was widely used for amino acid (AA) analysis, is no longer commercially available. Methods: To set up an affordable AA analysis program, a Beckman system gold HPLC 126AA analyzer and Pickering Laboratories reagents were used. Two quantitative AA analysis programs were developed. One was an 18-min short program quantitating seven AAs from plasma and dried blood spots (DBS) specimens using Lithium eluents Li-365 and Li-375 at 70°C column temperature. The short program could be used for diagnosis and follow-up dietary management for phenylketonuria (PKU), maple syrup urine disease (MSUD), tyrosinemia and homocystinuria patients. The second program was a 118-min long AA screening panel quantitating 40 AAs using Lithium eluents Li-275, Li-365 and Li-375 at 32, 48 and 72°C column temperatures from plasma and urine specimens. Results: The values obtained from DBS specimens were in good agreement with certified results from the Centers for Disease Control and Prevention. The values obtained from plasma and urine samples were in good correlation with those obtained from Beckman 6300 analyzer (0.9076≤r≤0.999). Conclusions: Amino acid quantitation from physiological samples using a Beckman 126AA Analyzer and Pickering Laboratories reagents was useful for clinical diagnosis and monitoring of aminoacidopathies.

Original languageEnglish
Pages (from-to)153-162
Number of pages10
JournalClinica Chimica Acta
Volume312
Issue number1-2
DOIs
Publication statusPublished - 10 Oct 2001

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Keywords

  • Amino acid analysis
  • Dot blot specimens
  • HPLC 126AA analyzer
  • PKU

Cite this

Qu, Y., Slocum, R. H., Fu, J., Rasmussen, W. E., Rector, H. D., Miller, J. B., & Coldwell, J. G. (2001). Quantitative amino acid analysis using a Beckman system gold HPLC 126AA analyzer. Clinica Chimica Acta, 312(1-2), 153-162. https://doi.org/10.1016/S0009-8981(01)00615-5