Abstract
Urinary tract infections (UTIs) cost $0.4-0.5 billion a year in the US and is the second most common disease affecting millions of people. As resistance to antibiotics becomes more common, a greater need for alternative treatments is needed. Nanodiamond particles (NDPs) are actively researched as drug delivery platforms due to their biocompatibility, particle size, and stable inert core. This research is aimed at developing NDPs as antibiotic drug delivery platforms for treating UTIs. To this end, 100 nm, 75 nm, 25 nm and 6 nm size NDPs are purified with acid and heat treatment techniques. Raman spectra of the NDPs showed that the acid treatment method resulted in higher diamond yield. Fourier transform infrared spectroscopy (FTIR) studies showed that both purification techniques result in oxygen terminated surface groups. Efficiency of loading amoxicillin on 25 nm NDPs based on electrostatic interaction of NDPs, functionalizing surfaces of NDPs with hydrogen, and polyethylenimine (PEI) are investigated. It is found that the electrostatic and surface hydrogenation approaches are not efficient in loading amoxicillin on the NDPs. On the other hand, PEI functionalized NDPs produced successful loading with amoxicillin as indicated by the presence of the β-lactam peak at 1770 cm-1, amide peak at 1680 cm-1, and bond between PEI NH stretching and amoxicillin -COOH group at 3650 cm-1 by the FTIR spectra. These results are expected to lay the foundation for developing NDP based targeted drug delivery treatment techniques for treating UTIs and other infectious diseases.
| Original language | English |
|---|---|
| Pages (from-to) | 323-332 |
| Number of pages | 10 |
| Journal | Materials Science and Engineering C |
| Volume | 63 |
| DOIs | |
| State | Published - 1 Jun 2016 |
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Keywords
- Amoxicillin
- Antibiotics
- Nanodiamond
- Polyethylenimine
- Targeted drug delivery
Cite this
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Purification and functionalization of nanodiamond to serve as a platform for amoxicillin delivery. / Rouhani, Parvaneh; Govindaraju, Nirmal; Iyer, Janaki K.; Kaul, Rashmi; Kaul, Anil; Singh, Raj N.
In: Materials Science and Engineering C, Vol. 63, 01.06.2016, p. 323-332.Research output: Contribution to journal › Article
TY - JOUR
T1 - Purification and functionalization of nanodiamond to serve as a platform for amoxicillin delivery
AU - Rouhani, Parvaneh
AU - Govindaraju, Nirmal
AU - Iyer, Janaki K.
AU - Kaul, Rashmi
AU - Kaul, Anil
AU - Singh, Raj N.
PY - 2016/6/1
Y1 - 2016/6/1
N2 - Urinary tract infections (UTIs) cost $0.4-0.5 billion a year in the US and is the second most common disease affecting millions of people. As resistance to antibiotics becomes more common, a greater need for alternative treatments is needed. Nanodiamond particles (NDPs) are actively researched as drug delivery platforms due to their biocompatibility, particle size, and stable inert core. This research is aimed at developing NDPs as antibiotic drug delivery platforms for treating UTIs. To this end, 100 nm, 75 nm, 25 nm and 6 nm size NDPs are purified with acid and heat treatment techniques. Raman spectra of the NDPs showed that the acid treatment method resulted in higher diamond yield. Fourier transform infrared spectroscopy (FTIR) studies showed that both purification techniques result in oxygen terminated surface groups. Efficiency of loading amoxicillin on 25 nm NDPs based on electrostatic interaction of NDPs, functionalizing surfaces of NDPs with hydrogen, and polyethylenimine (PEI) are investigated. It is found that the electrostatic and surface hydrogenation approaches are not efficient in loading amoxicillin on the NDPs. On the other hand, PEI functionalized NDPs produced successful loading with amoxicillin as indicated by the presence of the β-lactam peak at 1770 cm-1, amide peak at 1680 cm-1, and bond between PEI NH stretching and amoxicillin -COOH group at 3650 cm-1 by the FTIR spectra. These results are expected to lay the foundation for developing NDP based targeted drug delivery treatment techniques for treating UTIs and other infectious diseases.
AB - Urinary tract infections (UTIs) cost $0.4-0.5 billion a year in the US and is the second most common disease affecting millions of people. As resistance to antibiotics becomes more common, a greater need for alternative treatments is needed. Nanodiamond particles (NDPs) are actively researched as drug delivery platforms due to their biocompatibility, particle size, and stable inert core. This research is aimed at developing NDPs as antibiotic drug delivery platforms for treating UTIs. To this end, 100 nm, 75 nm, 25 nm and 6 nm size NDPs are purified with acid and heat treatment techniques. Raman spectra of the NDPs showed that the acid treatment method resulted in higher diamond yield. Fourier transform infrared spectroscopy (FTIR) studies showed that both purification techniques result in oxygen terminated surface groups. Efficiency of loading amoxicillin on 25 nm NDPs based on electrostatic interaction of NDPs, functionalizing surfaces of NDPs with hydrogen, and polyethylenimine (PEI) are investigated. It is found that the electrostatic and surface hydrogenation approaches are not efficient in loading amoxicillin on the NDPs. On the other hand, PEI functionalized NDPs produced successful loading with amoxicillin as indicated by the presence of the β-lactam peak at 1770 cm-1, amide peak at 1680 cm-1, and bond between PEI NH stretching and amoxicillin -COOH group at 3650 cm-1 by the FTIR spectra. These results are expected to lay the foundation for developing NDP based targeted drug delivery treatment techniques for treating UTIs and other infectious diseases.
KW - Amoxicillin
KW - Antibiotics
KW - Nanodiamond
KW - Polyethylenimine
KW - Targeted drug delivery
UR - http://www.scopus.com/inward/record.url?scp=84960333168&partnerID=8YFLogxK
U2 - 10.1016/j.msec.2016.02.075
DO - 10.1016/j.msec.2016.02.075
M3 - Article
C2 - 27040226
AN - SCOPUS:84960333168
VL - 63
SP - 323
EP - 332
JO - Materials Science and Engineering C
JF - Materials Science and Engineering C
SN - 0928-4931
ER -