Probiotic interference of lactobacillus rhamnosus GR-1 and lactobacillus reuteri RC-14 with the opportunistic fungal pathogen candida albicans

Gerwald Koehler, Senait Assefa, Gregor Reid

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64 Citations (Scopus)

Abstract

Candida albicans is the most important Candida species causing vulvovaginal candidiasis (VVC). VVC has significant medical and economical impact on womens health and wellbeing. While current antifungal treatment is reasonably effective, supportive and preventive measures such as application of probiotics are required to reduce the incidence of VVC. We investigated the potential of the probiotics Lactobacillus rhamnosus GR-1 and Lactobacillus reuteri RC-14 towards control of C. albicans. In vitro experiments demonstrated that lactic acid at low pH plays a major role in suppressing fungal growth. Viability staining following cocultures with lactobacilli revealed that C. albicans cells lost metabolic activity and eventually were killed. Transcriptome analyses showed increased expression of stress-related genes and lower expression of genes involved in fluconazole resistance, which might explain the increased eradication of Candida in a previous clinical study on conjoint probiotic therapy. Our results provide insights on the impact of probiotics on C. albicans survival.

Original languageEnglish
Article number636474
JournalInfectious Diseases in Obstetrics and Gynecology
Volume2012
DOIs
StatePublished - 1 Jan 2012

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Lactobacillus reuteri
Lactobacillus rhamnosus
Vulvovaginal Candidiasis
Probiotics
Candida albicans
Candida
Fluconazole
Gene Expression Profiling
Lactobacillus
Women's Health
Coculture Techniques
Lactic Acid
Staining and Labeling
Gene Expression
Incidence
Growth
Genes

Cite this

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title = "Probiotic interference of lactobacillus rhamnosus GR-1 and lactobacillus reuteri RC-14 with the opportunistic fungal pathogen candida albicans",
abstract = "Candida albicans is the most important Candida species causing vulvovaginal candidiasis (VVC). VVC has significant medical and economical impact on womens health and wellbeing. While current antifungal treatment is reasonably effective, supportive and preventive measures such as application of probiotics are required to reduce the incidence of VVC. We investigated the potential of the probiotics Lactobacillus rhamnosus GR-1 and Lactobacillus reuteri RC-14 towards control of C. albicans. In vitro experiments demonstrated that lactic acid at low pH plays a major role in suppressing fungal growth. Viability staining following cocultures with lactobacilli revealed that C. albicans cells lost metabolic activity and eventually were killed. Transcriptome analyses showed increased expression of stress-related genes and lower expression of genes involved in fluconazole resistance, which might explain the increased eradication of Candida in a previous clinical study on conjoint probiotic therapy. Our results provide insights on the impact of probiotics on C. albicans survival.",
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