Primate cytomegaloviruses encode and express an IL-10-like protein

Kristen M. Lockridge; Shan Shan Zhou; Rachel H. Kravitz; Jennifer L. Johnson; Earl T. Sawai; Earl L. Blewett; Peter A. Barry

doi:10.1006/viro.2000.0195

Primate cytomegaloviruses encode and express an IL-10-like protein

Kristen M. Lockridge, Shan Shan Zhou, Rachel H. Kravitz, Jennifer L. Johnson, Earl T. Sawai, Earl L. Blewett, Peter A. Barry

Biochemistry & Microbiology

Research output: Contribution to journal › Article › peer-review

142 Scopus citations

Abstract

An open reading frame (ORF) with homology to interleukin-10 (IL-10) has been identified in rhesus cytomegalovirus (RhCMV). The IL-10-like protein is generated from a multispliced, polyadenylated early gene transcript encompassing part of the corresponding UL111A ORF of human CMV (HCMV). Immunological analyses confirm expression of the IL-10-like protein both in tissue culture and in RhCMV-infected rhesus macaques. Conserved ORFs were subsequently identified in human, baboon, and African green monkey CMV, and a fully processed transcript has been mapped in fibroblasts infected with the Towne strain of HCMV. The conservation of this previously unrecognized ORF suggests that the protein may play an essential role in primate CMV persistence and pathogenesis. (C) 2000 Academic Press.

Original language	English
Pages (from-to)	272-280
Number of pages	9
Journal	Virology
Volume	268
Issue number	2
DOIs	https://doi.org/10.1006/viro.2000.0195
State	Published - 15 Mar 2000

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10.1006/viro.2000.0195

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title = "Primate cytomegaloviruses encode and express an IL-10-like protein",

abstract = "An open reading frame (ORF) with homology to interleukin-10 (IL-10) has been identified in rhesus cytomegalovirus (RhCMV). The IL-10-like protein is generated from a multispliced, polyadenylated early gene transcript encompassing part of the corresponding UL111A ORF of human CMV (HCMV). Immunological analyses confirm expression of the IL-10-like protein both in tissue culture and in RhCMV-infected rhesus macaques. Conserved ORFs were subsequently identified in human, baboon, and African green monkey CMV, and a fully processed transcript has been mapped in fibroblasts infected with the Towne strain of HCMV. The conservation of this previously unrecognized ORF suggests that the protein may play an essential role in primate CMV persistence and pathogenesis. (C) 2000 Academic Press.",

author = "Lockridge, {Kristen M.} and Zhou, {Shan Shan} and Kravitz, {Rachel H.} and Johnson, {Jennifer L.} and Sawai, {Earl T.} and Blewett, {Earl L.} and Barry, {Peter A.}",

note = "Funding Information: This work was supported by NIH Grants RO1 HL-57883 and RO1 NS-36859 (to P.A.B.) and by the base grant to the California Regional Primate Research Center (P51 RR-AG00169). The majority of this work was first presented at the 7th International Cytomegalovirus Workshop, Brighton, UK, May 1999.",

year = "2000",

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doi = "10.1006/viro.2000.0195",

language = "English",

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T1 - Primate cytomegaloviruses encode and express an IL-10-like protein

AU - Lockridge, Kristen M.

AU - Zhou, Shan Shan

AU - Kravitz, Rachel H.

AU - Johnson, Jennifer L.

AU - Sawai, Earl T.

AU - Blewett, Earl L.

AU - Barry, Peter A.

N1 - Funding Information: This work was supported by NIH Grants RO1 HL-57883 and RO1 NS-36859 (to P.A.B.) and by the base grant to the California Regional Primate Research Center (P51 RR-AG00169). The majority of this work was first presented at the 7th International Cytomegalovirus Workshop, Brighton, UK, May 1999.

PY - 2000/3/15

Y1 - 2000/3/15

N2 - An open reading frame (ORF) with homology to interleukin-10 (IL-10) has been identified in rhesus cytomegalovirus (RhCMV). The IL-10-like protein is generated from a multispliced, polyadenylated early gene transcript encompassing part of the corresponding UL111A ORF of human CMV (HCMV). Immunological analyses confirm expression of the IL-10-like protein both in tissue culture and in RhCMV-infected rhesus macaques. Conserved ORFs were subsequently identified in human, baboon, and African green monkey CMV, and a fully processed transcript has been mapped in fibroblasts infected with the Towne strain of HCMV. The conservation of this previously unrecognized ORF suggests that the protein may play an essential role in primate CMV persistence and pathogenesis. (C) 2000 Academic Press.

AB - An open reading frame (ORF) with homology to interleukin-10 (IL-10) has been identified in rhesus cytomegalovirus (RhCMV). The IL-10-like protein is generated from a multispliced, polyadenylated early gene transcript encompassing part of the corresponding UL111A ORF of human CMV (HCMV). Immunological analyses confirm expression of the IL-10-like protein both in tissue culture and in RhCMV-infected rhesus macaques. Conserved ORFs were subsequently identified in human, baboon, and African green monkey CMV, and a fully processed transcript has been mapped in fibroblasts infected with the Towne strain of HCMV. The conservation of this previously unrecognized ORF suggests that the protein may play an essential role in primate CMV persistence and pathogenesis. (C) 2000 Academic Press.

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VL - 268

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JO - Virology

JF - Virology

IS - 2

ER -

Primate cytomegaloviruses encode and express an IL-10-like protein

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