Preliminary crystallographic study of the Streptococcus agalactiae sortases, sortase A and sortase C1

Baldeep Khare, Alexandra Samal, Krishnan Vengadesan, K. R. Rajashankar, Xin Ma, I. Hsiu Huang, Hung Ton-That, Sthanam V.L. Narayana

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Sortases are cysteine transpeptidases that are essential for the assembly and anchoring of cell-surface adhesins in Gram-positive bacteria. In Streptococcus agalactiae (GBS), the pilin-specific sortase SrtC1 catalyzes the polymerization of pilins encoded by pilus island 1 (PI-1) and the housekeeping sortase SrtA is necessary for cell-wall anchoring of the resulting pilus polymers. These sortases are known to utilize different substrates for pilus polymerization and cell-wall anchoring; however, the structural correlates that dictate their substrate specificity have not yet been clearly defined. This report presents the expression, purification and crystallization of SrtC1 (SAG0647) and SrtA (SAG0961) from S. agalactiae strain 2603V/R. The GBS SrtC1 has been crystallized in three crystal forms and the GBS SrtA has been crystallized in one crystal form.

Original languageEnglish
Pages (from-to)1096-1100
Number of pages5
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume66
Issue number9
DOIs
StatePublished - 1 Sep 2010

Keywords

  • sortase A
  • sortase C1
  • sortases
  • Streptococcus agalactiae

Fingerprint Dive into the research topics of 'Preliminary crystallographic study of the Streptococcus agalactiae sortases, sortase A and sortase C1'. Together they form a unique fingerprint.

  • Cite this