Phosphoinositides and inositol phosphates in Discopyge tschudii electrocyte membranes

H. R. Arias, F. J. Barrantes

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

1. 1. The metabolism of inositol (Ins)-containing phospholipids and inositol phosphates has been studied by following the incorporation and distribution of myo-[3 H]Ins in metabolically active electrocytes from the electric ray Discopyge tschudii. 2. 2. The apparent initial rate of myo-[3H]Ins incorporation into total phosphoinositides was ca 8.2 fmol/mg protein/hr. Phosphatidylinositol (Ptdlns) displayed the highest levels of labelling. Lithium inhibited this incorporation probably by limiting the recycling of myo-[3H]Ins from [3H]Ins-monophosphate. 3. 3. The formation of water-soluble products of phosphoinositides between 7 and 24 hr was 4.1 ± 0.2, 0.4 ± 0.2 and 3.0 ± 1.0 fmol/μmmol total lipid phosphorus for myo-[3H]InsP, -InsP2 and Ins-P3 respectively. 4. 4. Lithium ions are shown to modulate phosphoinositide synthesis and Ins-phosphate accumulation. Ins-mono-, bis- and tris-phosphate production was enhanced 5-, 3- and 2-fold by Li +. 5. 5. The above results suggest the participation of a C-type phospholipase and of Li-sensitive phosphatases in the modulation of phosphoinositide metabolism in the electrocyte.

Original languageEnglish
Pages (from-to)1387-1392
Number of pages6
JournalInternational Journal of Biochemistry
Volume22
Issue number12
DOIs
StatePublished - 1 Jan 1990
Externally publishedYes

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Inositol Phosphates
Inositol
Phosphatidylinositols
Membranes
Lithium
Metabolism
Phosphates
Torpedo
Recycling
Type C Phospholipases
Phosphoric Monoester Hydrolases
Phospholipids
Phosphorus
Labeling
Ions
Lipids
Modulation
Water
Proteins

Cite this

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title = "Phosphoinositides and inositol phosphates in Discopyge tschudii electrocyte membranes",
abstract = "1. 1. The metabolism of inositol (Ins)-containing phospholipids and inositol phosphates has been studied by following the incorporation and distribution of myo-[3 H]Ins in metabolically active electrocytes from the electric ray Discopyge tschudii. 2. 2. The apparent initial rate of myo-[3H]Ins incorporation into total phosphoinositides was ca 8.2 fmol/mg protein/hr. Phosphatidylinositol (Ptdlns) displayed the highest levels of labelling. Lithium inhibited this incorporation probably by limiting the recycling of myo-[3H]Ins from [3H]Ins-monophosphate. 3. 3. The formation of water-soluble products of phosphoinositides between 7 and 24 hr was 4.1 ± 0.2, 0.4 ± 0.2 and 3.0 ± 1.0 fmol/μmmol total lipid phosphorus for myo-[3H]InsP, -InsP2 and Ins-P3 respectively. 4. 4. Lithium ions are shown to modulate phosphoinositide synthesis and Ins-phosphate accumulation. Ins-mono-, bis- and tris-phosphate production was enhanced 5-, 3- and 2-fold by Li +. 5. 5. The above results suggest the participation of a C-type phospholipase and of Li-sensitive phosphatases in the modulation of phosphoinositide metabolism in the electrocyte.",
author = "Arias, {H. R.} and Barrantes, {F. J.}",
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Phosphoinositides and inositol phosphates in Discopyge tschudii electrocyte membranes. / Arias, H. R.; Barrantes, F. J.

In: International Journal of Biochemistry, Vol. 22, No. 12, 01.01.1990, p. 1387-1392.

Research output: Contribution to journalArticle

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AU - Arias, H. R.

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N2 - 1. 1. The metabolism of inositol (Ins)-containing phospholipids and inositol phosphates has been studied by following the incorporation and distribution of myo-[3 H]Ins in metabolically active electrocytes from the electric ray Discopyge tschudii. 2. 2. The apparent initial rate of myo-[3H]Ins incorporation into total phosphoinositides was ca 8.2 fmol/mg protein/hr. Phosphatidylinositol (Ptdlns) displayed the highest levels of labelling. Lithium inhibited this incorporation probably by limiting the recycling of myo-[3H]Ins from [3H]Ins-monophosphate. 3. 3. The formation of water-soluble products of phosphoinositides between 7 and 24 hr was 4.1 ± 0.2, 0.4 ± 0.2 and 3.0 ± 1.0 fmol/μmmol total lipid phosphorus for myo-[3H]InsP, -InsP2 and Ins-P3 respectively. 4. 4. Lithium ions are shown to modulate phosphoinositide synthesis and Ins-phosphate accumulation. Ins-mono-, bis- and tris-phosphate production was enhanced 5-, 3- and 2-fold by Li +. 5. 5. The above results suggest the participation of a C-type phospholipase and of Li-sensitive phosphatases in the modulation of phosphoinositide metabolism in the electrocyte.

AB - 1. 1. The metabolism of inositol (Ins)-containing phospholipids and inositol phosphates has been studied by following the incorporation and distribution of myo-[3 H]Ins in metabolically active electrocytes from the electric ray Discopyge tschudii. 2. 2. The apparent initial rate of myo-[3H]Ins incorporation into total phosphoinositides was ca 8.2 fmol/mg protein/hr. Phosphatidylinositol (Ptdlns) displayed the highest levels of labelling. Lithium inhibited this incorporation probably by limiting the recycling of myo-[3H]Ins from [3H]Ins-monophosphate. 3. 3. The formation of water-soluble products of phosphoinositides between 7 and 24 hr was 4.1 ± 0.2, 0.4 ± 0.2 and 3.0 ± 1.0 fmol/μmmol total lipid phosphorus for myo-[3H]InsP, -InsP2 and Ins-P3 respectively. 4. 4. Lithium ions are shown to modulate phosphoinositide synthesis and Ins-phosphate accumulation. Ins-mono-, bis- and tris-phosphate production was enhanced 5-, 3- and 2-fold by Li +. 5. 5. The above results suggest the participation of a C-type phospholipase and of Li-sensitive phosphatases in the modulation of phosphoinositide metabolism in the electrocyte.

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