Peptostreptococcus canis sp. nov., isolated from subgingival plaque from canine oral cavity

Paul A. Lawson; Crystal N. Johnson; Lisbeth Bengtsson; Georgios Charalampakis; Gunnar Dahlén; Edward Moore; Enevold Falsen

doi:10.1016/j.anaerobe.2012.10.008

Peptostreptococcus canis sp. nov., isolated from subgingival plaque from canine oral cavity

Paul A. Lawson, Crystal N. Johnson, Lisbeth Bengtsson, Georgios Charalampakis, Gunnar Dahlén, Edward Moore, Enevold Falsen

Oklahoma State University Center for Health Sciences

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

A polyphasic taxonomic study was performed on two strains of an unknown Gram-positive, asaccharolytic, nonspore-forming, obligately anaerobic coccus-shaped bacterium isolated from oral subgingival plaque of Labrador retriever dogs. Comparative 16S rRNA gene sequencing confirmed that these isolates were highly related to each other and formed a hitherto unknown linage within the clostridial rRNA XI cluster of organisms. Pairwise analysis demonstrated that the novel organism to be most closely related to members of the genus Peptostreptococcus with 16S rDNA gene sequence similarity values between 92.8% and 96.7%, respectively. The G + C DNA base composition was 30.8 mol% and the major cellular fatty acids included iso-C_14:0, iso-C_16:0, and iso-C_{16:0 DMA}. Based on biochemical, chemotaxonomic, and phylogenetic evidence it is proposed that the unknown bacterium be classified as a new species, Peptostreptococcus canis sp. nov. The type strain is CCUG 57081^T.

Original language	English
Pages (from-to)	597-601
Number of pages	5
Journal	Anaerobe
Volume	18
Issue number	6
DOIs	https://doi.org/10.1016/j.anaerobe.2012.10.008
State	Published - Dec 2012

Keywords

16S RNA
Canine
CCUG
Peptostreptococci
Phylogeny
Taxonomy

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10.1016/j.anaerobe.2012.10.008

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title = "Peptostreptococcus canis sp. nov., isolated from subgingival plaque from canine oral cavity",

abstract = "A polyphasic taxonomic study was performed on two strains of an unknown Gram-positive, asaccharolytic, nonspore-forming, obligately anaerobic coccus-shaped bacterium isolated from oral subgingival plaque of Labrador retriever dogs. Comparative 16S rRNA gene sequencing confirmed that these isolates were highly related to each other and formed a hitherto unknown linage within the clostridial rRNA XI cluster of organisms. Pairwise analysis demonstrated that the novel organism to be most closely related to members of the genus Peptostreptococcus with 16S rDNA gene sequence similarity values between 92.8% and 96.7%, respectively. The G + C DNA base composition was 30.8 mol% and the major cellular fatty acids included iso-C14:0, iso-C16:0, and iso-C16:0 DMA. Based on biochemical, chemotaxonomic, and phylogenetic evidence it is proposed that the unknown bacterium be classified as a new species, Peptostreptococcus canis sp. nov. The type strain is CCUG 57081T.",

keywords = "16S RNA, Canine, CCUG, Peptostreptococci, Phylogeny, Taxonomy",

author = "Lawson, {Paul A.} and Johnson, {Crystal N.} and Lisbeth Bengtsson and Georgios Charalampakis and Gunnar Dahl{\'e}n and Edward Moore and Enevold Falsen",

year = "2012",

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doi = "10.1016/j.anaerobe.2012.10.008",

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AU - Lawson, Paul A.

AU - Johnson, Crystal N.

AU - Bengtsson, Lisbeth

AU - Charalampakis, Georgios

AU - Dahlén, Gunnar

AU - Moore, Edward

AU - Falsen, Enevold

PY - 2012/12

Y1 - 2012/12

N2 - A polyphasic taxonomic study was performed on two strains of an unknown Gram-positive, asaccharolytic, nonspore-forming, obligately anaerobic coccus-shaped bacterium isolated from oral subgingival plaque of Labrador retriever dogs. Comparative 16S rRNA gene sequencing confirmed that these isolates were highly related to each other and formed a hitherto unknown linage within the clostridial rRNA XI cluster of organisms. Pairwise analysis demonstrated that the novel organism to be most closely related to members of the genus Peptostreptococcus with 16S rDNA gene sequence similarity values between 92.8% and 96.7%, respectively. The G + C DNA base composition was 30.8 mol% and the major cellular fatty acids included iso-C14:0, iso-C16:0, and iso-C16:0 DMA. Based on biochemical, chemotaxonomic, and phylogenetic evidence it is proposed that the unknown bacterium be classified as a new species, Peptostreptococcus canis sp. nov. The type strain is CCUG 57081T.

AB - A polyphasic taxonomic study was performed on two strains of an unknown Gram-positive, asaccharolytic, nonspore-forming, obligately anaerobic coccus-shaped bacterium isolated from oral subgingival plaque of Labrador retriever dogs. Comparative 16S rRNA gene sequencing confirmed that these isolates were highly related to each other and formed a hitherto unknown linage within the clostridial rRNA XI cluster of organisms. Pairwise analysis demonstrated that the novel organism to be most closely related to members of the genus Peptostreptococcus with 16S rDNA gene sequence similarity values between 92.8% and 96.7%, respectively. The G + C DNA base composition was 30.8 mol% and the major cellular fatty acids included iso-C14:0, iso-C16:0, and iso-C16:0 DMA. Based on biochemical, chemotaxonomic, and phylogenetic evidence it is proposed that the unknown bacterium be classified as a new species, Peptostreptococcus canis sp. nov. The type strain is CCUG 57081T.

KW - 16S RNA

KW - Canine

KW - CCUG

KW - Peptostreptococci

KW - Phylogeny

KW - Taxonomy

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DO - 10.1016/j.anaerobe.2012.10.008

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SN - 1075-9964

VL - 18

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EP - 601

JO - Anaerobe

JF - Anaerobe

IS - 6

ER -

Peptostreptococcus canis sp. nov., isolated from subgingival plaque from canine oral cavity

Abstract

Keywords

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