Nicotine smoking concentrations modulate GABAergic synaptic transmission in murine medial prefrontal cortex by activation of α7* and β2* nicotinic receptors

Roberto Cuevas-Olguin, Eric Esquivel-Rendon, Jorge Vargas-Mireles, Carlos Barajas-Lόpez, Roberto Salgado-Delgado, Nadia Saderi, Hugo R. Arias, Marco Atzori, Marcela Miranda-Morales

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Nicotine is the major addictive component of cigarettes, reaching a brain concentration of ~300 nM during smoking of a single cigarette. The prefrontal cortex (PFC) mechanisms underlying temporary changes of working memory during smoking are incompletely understood. Here, we investigated whether 300 nM nicotine modulates γ-aminobutyric acid (GABA) ergic synaptic transmission from pyramidal neurons of the output layer (V) of the murine medial PFC. We used patch clamp in vitro recording from C57BL/6 mice in the whole-cell configuration to investigate the effect of nicotine on pharmacologically isolated GABAergic postsynaptic currents (IPSCs) in the absence or presence of methyllycaconitine (MLA) or dihydro-β-erythroidine (DHβE), selective antagonists of α7- and β2-containing (α7* and β2*) nicotinic acetylcholine receptors (AChRs), respectively. Our results indicated that nicotine, alone or in the presence of MLA, decreases electrically evoked IPSC (eIPSC) amplitude, whereas in the presence of DHβE, nicotine elicited either an eIPSCs amplitude increase or a decrease. In the presence of DHβE, nicotine increased membrane conductance leaving the paired pulse ratio unchanged in all conditions, suggesting a non-β2* mediated effect. In the presence of MLA, nicotine decreased the mean spontaneous IPSC (sIPSC) frequency but increased their rise time, suggesting a non-α7* AChR-mediated synaptic modulation. Also, in the presence of DHβE, nicotine decreased both eIPSC rise and decay times. No receptors other than α7* and β2* appear to be involved in the nicotine effect. Our results indicate that nicotine smoking concentrations modulate GABAergic synaptic currents through mixed pre- and post-synaptic mechanisms by activation of α7* and β2* AChRs.

Original languageEnglish
Pages (from-to)781-792
Number of pages12
JournalEuropean Journal of Neuroscience
Volume51
Issue number3
DOIs
StatePublished - 1 Feb 2020

Keywords

  • central nervous system
  • gamma-aminobutyric acid
  • pair pulse
  • patch clamp

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