New molecular methods to study gene functions in Candida infections

S. Theiß, Gerwald Koehler, M. Kretschmar, T. Nichterlein, J. Hacker

Research output: Contribution to journalReview article

5 Citations (Scopus)

Abstract

Summary. Candida albicans has become a model system for human pathogenic fungi in clinical research, mainly due to the increasing number of Candida infections. Molecular techniques to study C. albicans virulence properties have been improved over the last few years, despite difficulties in genetic manipulation of this fungus. Some of the recent achievements from our own laboratory or from other groups are described in this article. The molecular analysis of the recently identified ATP-dependent transporter Mlt1 using the green fluorescent protein (GFP) as reporter for protein localization and the dominant MPAR gene as a selection marker for gene inactivation provides an example for the study of gene functions in C. albicans.

Original languageEnglish
Pages (from-to)345-350
Number of pages6
JournalMycoses
Volume45
Issue number9-10
DOIs
StatePublished - 30 Nov 2002

Fingerprint

candidiasis
Candida albicans
Candida
animal pathogenic fungi
Fungi
Infection
Genes
Dominant Genes
genes
dominant genes
Gene Silencing
Green Fluorescent Proteins
green fluorescent protein
genetic engineering
Virulence
transporters
inactivation
virulence
Adenosine Triphosphate
methodology

Keywords

  • ABC-transporter
  • Candida
  • Gene expression
  • Gene function
  • Molecular genetics

Cite this

Theiß, S. ; Koehler, Gerwald ; Kretschmar, M. ; Nichterlein, T. ; Hacker, J. / New molecular methods to study gene functions in Candida infections. In: Mycoses. 2002 ; Vol. 45, No. 9-10. pp. 345-350.
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Theiß, S, Koehler, G, Kretschmar, M, Nichterlein, T & Hacker, J 2002, 'New molecular methods to study gene functions in Candida infections', Mycoses, vol. 45, no. 9-10, pp. 345-350. https://doi.org/10.1046/j.1439-0507.2002.00792.x

New molecular methods to study gene functions in Candida infections. / Theiß, S.; Koehler, Gerwald; Kretschmar, M.; Nichterlein, T.; Hacker, J.

In: Mycoses, Vol. 45, No. 9-10, 30.11.2002, p. 345-350.

Research output: Contribution to journalReview article

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T1 - New molecular methods to study gene functions in Candida infections

AU - Theiß, S.

AU - Koehler, Gerwald

AU - Kretschmar, M.

AU - Nichterlein, T.

AU - Hacker, J.

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AB - Summary. Candida albicans has become a model system for human pathogenic fungi in clinical research, mainly due to the increasing number of Candida infections. Molecular techniques to study C. albicans virulence properties have been improved over the last few years, despite difficulties in genetic manipulation of this fungus. Some of the recent achievements from our own laboratory or from other groups are described in this article. The molecular analysis of the recently identified ATP-dependent transporter Mlt1 using the green fluorescent protein (GFP) as reporter for protein localization and the dominant MPAR gene as a selection marker for gene inactivation provides an example for the study of gene functions in C. albicans.

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