Abstract
A microplate-format assay for measurement of histamine release by activated rat peritoneal mast cells, with accompanying microplate-format spectrofluorometric assay of histamine, is demonstrated. Reproducibility and linearity of the histamine assay are comparable to the historical large format assay. Transfer of samples in the 96-well format, from histamine release through spectrofluorometric assay, greatly expedites handling and provides for better independence of each replicate. A sample experiment is included to show a typical size experiment easily done in this format.
| Original language | English |
|---|---|
| Pages (from-to) | 958-962 |
| Number of pages | 5 |
| Journal | BioTechniques |
| Volume | 22 |
| Issue number | 5 |
| DOIs | |
| State | Published - May 1997 |