Microplate assay for measurement of histamine release from mast cells

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Abstract

A microplate-format assay for measurement of histamine release by activated rat peritoneal mast cells, with accompanying microplate-format spectrofluorometric assay of histamine, is demonstrated. Reproducibility and linearity of the histamine assay are comparable to the historical large format assay. Transfer of samples in the 96-well format, from histamine release through spectrofluorometric assay, greatly expedites handling and provides for better independence of each replicate. A sample experiment is included to show a typical size experiment easily done in this format.

Original languageEnglish
Pages (from-to)958-962
Number of pages5
JournalBioTechniques
Volume22
Issue number5
StatePublished - 1 May 1997

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Histamine Release
Mast Cells
Histamine
Assays
Rats
Experiments

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abstract = "A microplate-format assay for measurement of histamine release by activated rat peritoneal mast cells, with accompanying microplate-format spectrofluorometric assay of histamine, is demonstrated. Reproducibility and linearity of the histamine assay are comparable to the historical large format assay. Transfer of samples in the 96-well format, from histamine release through spectrofluorometric assay, greatly expedites handling and provides for better independence of each replicate. A sample experiment is included to show a typical size experiment easily done in this format.",
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Microplate assay for measurement of histamine release from mast cells. / Price, Joseph A.

In: BioTechniques, Vol. 22, No. 5, 01.05.1997, p. 958-962.

Research output: Contribution to journalArticle

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AB - A microplate-format assay for measurement of histamine release by activated rat peritoneal mast cells, with accompanying microplate-format spectrofluorometric assay of histamine, is demonstrated. Reproducibility and linearity of the histamine assay are comparable to the historical large format assay. Transfer of samples in the 96-well format, from histamine release through spectrofluorometric assay, greatly expedites handling and provides for better independence of each replicate. A sample experiment is included to show a typical size experiment easily done in this format.

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