Mast cell degranulating peptide binds to RBL-2H3 mast cell receptors and inhibits IgE binding

Angeliki Buku, Joseph Price, Milton Mendlowitz, Sandra Masur

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Fluorescent and biotinylated analogs of mast cell degranulating (MCD) peptide were synthesized and the labels fluoresceinisothiocyanate and N-hydroxysuccinimidobiotin were conjugated at position 1 in the MCD peptide sequence. The analogs with these moieties retained histamine-releasing activity as high as that of the parent MCD peptide in rat peritoneal mast cell assays. These labeled analogs were used in rat basophilic leukemia cells (RBL-2H3) to demonstrate by confocal microscopy and flow cytometry the specific binding of MCD peptide to mast cell receptors. Consequently MCD peptide was found to compete with and inhibit the binding of fluorescent IgE on RBL cells as monitored by flow cytometry. Thus MCD peptide may prove to be useful in the study of IgE receptor-bearing cells.

Original languageEnglish
Pages (from-to)1993-1998
Number of pages6
JournalPeptides
Volume22
Issue number12
DOIs
StatePublished - 1 Jan 2001

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IgE Receptors
Mast Cells
Immunoglobulin E
Flow cytometry
Rats
Flow Cytometry
Bearings (structural)
Confocal microscopy
Confocal Microscopy
Histamine
mast cell degranulating peptide
Labels
Assays
Leukemia

Cite this

Buku, Angeliki ; Price, Joseph ; Mendlowitz, Milton ; Masur, Sandra. / Mast cell degranulating peptide binds to RBL-2H3 mast cell receptors and inhibits IgE binding. In: Peptides. 2001 ; Vol. 22, No. 12. pp. 1993-1998.
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Mast cell degranulating peptide binds to RBL-2H3 mast cell receptors and inhibits IgE binding. / Buku, Angeliki; Price, Joseph; Mendlowitz, Milton; Masur, Sandra.

In: Peptides, Vol. 22, No. 12, 01.01.2001, p. 1993-1998.

Research output: Contribution to journalArticle

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