Lumbar dorsal root ganglia of the cat: A quantitative study of peptide immunoreactivity and cell size

M. G. Garry, Kenneth Miller, V. S. Seybold

Research output: Contribution to journalArticle

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Abstract

The purpose of the present study was to quantify the extent to which several peptides and serotonin coexist with substance P or somatostatin in selected lumbar dorsal root ganglia of the cat. The technique for the simultaneous visualization of two antigens by immunofluorescence was used to investigate the coexistence of neuropeptides in the lumbar dorsal root ganglia of colchicine‐treated cats. Perikarya immunoreactive for calcitonin gene‐related peptide, galanin, leu‐enkephalin, somatostatin, and substance P were visualized in both the lumbar 5 and 6 dorsal root ganglia. In contrast, no immunoreactivity was observed for adipokinetic hormone, bombesin, dynorphin A, met‐enkephalin, oxytocin, tyrosine hydroxylase, thyrotropin‐releasing hormone, vasopressin, vasoactive intestinal peptide, or serotonin in either ganglion examined. Substance P coexisted with calcitonin‐gene‐related peptide, somatostatin, and leu‐enkephalin. Somatostatin was colocalized with calcitonin gene‐related peptide, leu‐enkephalin, and substance P but coexisted with galanin minimally. The cell area of immunoreactive perikarya was also examined. Data concerning the cross‐sectional area of immunoreactive cells indicated that somatostatin‐immunoreactive perikarya were generally the largest population observed (up to ∼6,000 μm2). Somatostatin and calcitonin gene‐related peptide, as well as substance P and calcitonin gene‐related peptide, coexisted in populations of cell bodies that had a smaller size (less than 2,000 μm2). These results suggest that certain peptides which coexist in the dorsal root ganglia may provide histochemical markers for functional groups of primary afferent neurons.

Original languageEnglish
Pages (from-to)36-47
Number of pages12
JournalJournal of Comparative Neurology
Volume284
Issue number1
DOIs
StatePublished - 1 Jan 1989

Fingerprint

Spinal Ganglia
Substance P
Somatostatin
Cell Size
Calcitonin
Cats
Peptides
Galanin
Serotonin
Dynorphins
Afferent Neurons
Bombesin
Vasoactive Intestinal Peptide
Tyrosine 3-Monooxygenase
Oxytocin
Neuropeptides
Vasopressins
Ganglia
Population
Fluorescent Antibody Technique

Keywords

  • cell area
  • coexistence of peptides
  • neuropeptides
  • primary afferent neuron

Cite this

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abstract = "The purpose of the present study was to quantify the extent to which several peptides and serotonin coexist with substance P or somatostatin in selected lumbar dorsal root ganglia of the cat. The technique for the simultaneous visualization of two antigens by immunofluorescence was used to investigate the coexistence of neuropeptides in the lumbar dorsal root ganglia of colchicine‐treated cats. Perikarya immunoreactive for calcitonin gene‐related peptide, galanin, leu‐enkephalin, somatostatin, and substance P were visualized in both the lumbar 5 and 6 dorsal root ganglia. In contrast, no immunoreactivity was observed for adipokinetic hormone, bombesin, dynorphin A, met‐enkephalin, oxytocin, tyrosine hydroxylase, thyrotropin‐releasing hormone, vasopressin, vasoactive intestinal peptide, or serotonin in either ganglion examined. Substance P coexisted with calcitonin‐gene‐related peptide, somatostatin, and leu‐enkephalin. Somatostatin was colocalized with calcitonin gene‐related peptide, leu‐enkephalin, and substance P but coexisted with galanin minimally. The cell area of immunoreactive perikarya was also examined. Data concerning the cross‐sectional area of immunoreactive cells indicated that somatostatin‐immunoreactive perikarya were generally the largest population observed (up to ∼6,000 μm2). Somatostatin and calcitonin gene‐related peptide, as well as substance P and calcitonin gene‐related peptide, coexisted in populations of cell bodies that had a smaller size (less than 2,000 μm2). These results suggest that certain peptides which coexist in the dorsal root ganglia may provide histochemical markers for functional groups of primary afferent neurons.",
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Lumbar dorsal root ganglia of the cat : A quantitative study of peptide immunoreactivity and cell size. / Garry, M. G.; Miller, Kenneth; Seybold, V. S.

In: Journal of Comparative Neurology, Vol. 284, No. 1, 01.01.1989, p. 36-47.

Research output: Contribution to journalArticle

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