Localization of estrogen receptor protein and estrogen receptor messenger RNA in peripheral autonomic and sensory neurons

R. E. Papka, B. Srinivasan, Kenneth Miller, S. Hayashi

Research output: Contribution to journalArticle

99 Citations (Scopus)

Abstract

The presence of estrogen receptor protein and estrogen receptor messenger RNA was revealed in peripheral ganglionic neurons of the rat. The pelvic parasympathetic autonomic ganglion and lumbosacral dorsal root sensory ganglia were examined for estrogen receptor-containing neurons because they have known projections to the uterus and uterine cervix. The vagal nodose ganglia were studied for estrogen receptor-containing neurons because they are suspected sources of influence on the uterus. Immunohistochemistry, in situ hybridization histochemistry and retrograde tracing were utilized. Immunoreactivity for estrogen receptors was evident in the nuclei of a subpopulation of neurons in the pelvic ganglia, sixth lumbar and first sacral dorsal root ganglia and nodose ganglia. Some estrogen receptor-positive neurons also contained the retrograde tracer FluoroGold that previously had been injected into the uterus and uterine cervix. Estrogen receptor messenger RNA was also evident in a subpopulation of ganglionic neurons. These data suggest that a certain population of neurons in autonomic and sensory ganglia are capable of synthesizing estrogen receptors and these receptors can serve as binding sites for estrogen. Thus, certain aspects of the structure, function and neurochemistry of some autonomic and sensory neurons may be influenced by the sex steroid estrogen.

Original languageEnglish
Pages (from-to)1153-1163
Number of pages11
JournalNeuroscience
Volume79
Issue number4
DOIs
StatePublished - 6 Jun 1997

Fingerprint

Sensory Receptor Cells
Estrogen Receptors
Neurons
Messenger RNA
Autonomic Ganglia
Nodose Ganglion
Sensory Ganglia
Uterus
Spinal Ganglia
Cervix Uteri
Estrogens
Parasympathetic Ganglia
Neurochemistry
estrophilin
Ganglia
In Situ Hybridization
Immunohistochemistry
Steroids
Binding Sites
Population

Keywords

  • Dorsal root ganglia
  • Estrogen receptors
  • Nodose ganglia
  • Pelvic ganglia
  • Sensory neurons
  • Vagus

Cite this

@article{622eb1688f1f4cb3ad5eea427f903898,
title = "Localization of estrogen receptor protein and estrogen receptor messenger RNA in peripheral autonomic and sensory neurons",
abstract = "The presence of estrogen receptor protein and estrogen receptor messenger RNA was revealed in peripheral ganglionic neurons of the rat. The pelvic parasympathetic autonomic ganglion and lumbosacral dorsal root sensory ganglia were examined for estrogen receptor-containing neurons because they have known projections to the uterus and uterine cervix. The vagal nodose ganglia were studied for estrogen receptor-containing neurons because they are suspected sources of influence on the uterus. Immunohistochemistry, in situ hybridization histochemistry and retrograde tracing were utilized. Immunoreactivity for estrogen receptors was evident in the nuclei of a subpopulation of neurons in the pelvic ganglia, sixth lumbar and first sacral dorsal root ganglia and nodose ganglia. Some estrogen receptor-positive neurons also contained the retrograde tracer FluoroGold that previously had been injected into the uterus and uterine cervix. Estrogen receptor messenger RNA was also evident in a subpopulation of ganglionic neurons. These data suggest that a certain population of neurons in autonomic and sensory ganglia are capable of synthesizing estrogen receptors and these receptors can serve as binding sites for estrogen. Thus, certain aspects of the structure, function and neurochemistry of some autonomic and sensory neurons may be influenced by the sex steroid estrogen.",
keywords = "Dorsal root ganglia, Estrogen receptors, Nodose ganglia, Pelvic ganglia, Sensory neurons, Vagus",
author = "Papka, {R. E.} and B. Srinivasan and Kenneth Miller and S. Hayashi",
year = "1997",
month = "6",
day = "6",
doi = "10.1016/S0306-4522(97)00076-6",
language = "English",
volume = "79",
pages = "1153--1163",
journal = "Neuroscience",
issn = "0306-4522",
publisher = "Elsevier Ltd",
number = "4",

}

Localization of estrogen receptor protein and estrogen receptor messenger RNA in peripheral autonomic and sensory neurons. / Papka, R. E.; Srinivasan, B.; Miller, Kenneth; Hayashi, S.

In: Neuroscience, Vol. 79, No. 4, 06.06.1997, p. 1153-1163.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Localization of estrogen receptor protein and estrogen receptor messenger RNA in peripheral autonomic and sensory neurons

AU - Papka, R. E.

AU - Srinivasan, B.

AU - Miller, Kenneth

AU - Hayashi, S.

PY - 1997/6/6

Y1 - 1997/6/6

N2 - The presence of estrogen receptor protein and estrogen receptor messenger RNA was revealed in peripheral ganglionic neurons of the rat. The pelvic parasympathetic autonomic ganglion and lumbosacral dorsal root sensory ganglia were examined for estrogen receptor-containing neurons because they have known projections to the uterus and uterine cervix. The vagal nodose ganglia were studied for estrogen receptor-containing neurons because they are suspected sources of influence on the uterus. Immunohistochemistry, in situ hybridization histochemistry and retrograde tracing were utilized. Immunoreactivity for estrogen receptors was evident in the nuclei of a subpopulation of neurons in the pelvic ganglia, sixth lumbar and first sacral dorsal root ganglia and nodose ganglia. Some estrogen receptor-positive neurons also contained the retrograde tracer FluoroGold that previously had been injected into the uterus and uterine cervix. Estrogen receptor messenger RNA was also evident in a subpopulation of ganglionic neurons. These data suggest that a certain population of neurons in autonomic and sensory ganglia are capable of synthesizing estrogen receptors and these receptors can serve as binding sites for estrogen. Thus, certain aspects of the structure, function and neurochemistry of some autonomic and sensory neurons may be influenced by the sex steroid estrogen.

AB - The presence of estrogen receptor protein and estrogen receptor messenger RNA was revealed in peripheral ganglionic neurons of the rat. The pelvic parasympathetic autonomic ganglion and lumbosacral dorsal root sensory ganglia were examined for estrogen receptor-containing neurons because they have known projections to the uterus and uterine cervix. The vagal nodose ganglia were studied for estrogen receptor-containing neurons because they are suspected sources of influence on the uterus. Immunohistochemistry, in situ hybridization histochemistry and retrograde tracing were utilized. Immunoreactivity for estrogen receptors was evident in the nuclei of a subpopulation of neurons in the pelvic ganglia, sixth lumbar and first sacral dorsal root ganglia and nodose ganglia. Some estrogen receptor-positive neurons also contained the retrograde tracer FluoroGold that previously had been injected into the uterus and uterine cervix. Estrogen receptor messenger RNA was also evident in a subpopulation of ganglionic neurons. These data suggest that a certain population of neurons in autonomic and sensory ganglia are capable of synthesizing estrogen receptors and these receptors can serve as binding sites for estrogen. Thus, certain aspects of the structure, function and neurochemistry of some autonomic and sensory neurons may be influenced by the sex steroid estrogen.

KW - Dorsal root ganglia

KW - Estrogen receptors

KW - Nodose ganglia

KW - Pelvic ganglia

KW - Sensory neurons

KW - Vagus

UR - http://www.scopus.com/inward/record.url?scp=0030744323&partnerID=8YFLogxK

U2 - 10.1016/S0306-4522(97)00076-6

DO - 10.1016/S0306-4522(97)00076-6

M3 - Article

C2 - 9219974

AN - SCOPUS:0030744323

VL - 79

SP - 1153

EP - 1163

JO - Neuroscience

JF - Neuroscience

SN - 0306-4522

IS - 4

ER -