Lipid-protein interactions and effect of local anesthetics in acetylcholine receptor-rich membranes from Torpedo marmorata electric organ

S. B. Mantipragada, L. I. Horváth, H. R. Arias, G. Schwarzmann, K. Sandhoff, F. J. Barrantes, D. Marsh

Research output: Contribution to journalArticle

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Abstract

The selectivity of lipid-protein interaction for spin-labeled phospholipids and gangliosides in nicotinic acetylcholine receptor-rich membranes from Torpedo marmorata has been studied by ESR spectroscopy. The association constants of the spin-labeled lipids (relative to phosphatidylcholine) at Ph 8.0 are in the order cardiolipin (5.1) ≈ stearic acid (4.9) ≈ phosphatidylinositol (4.7) > phosphatidylserine (2.7) > phosphatidylglycerol (1.7) > GD1b ≈ GM1 ≈ GM2 ≈ GM3 ≈ phosphatidylcholine (1.0) > phosphatidylethanolamine (0.5). No selectivity for mono- or disialogangliosides is found over that for phosphatidylcholine. Aminated local anesthetics were found to compete with spin-labeled phosphatidylinositol, but to a much lesser extent with spin-labeled stearic acid, for sites on the intramembranous surface of the protein. The relative association constant of phosphatidylinositol was reduced in the presence of the different local anesthetics to the following extents: tetracaine (55%) > procaine (35%) ≈ benzocaine (30%). For stearic acid, only tetracaine gave an appreciable reduction (30%) in association constant. These displacements represent an intrinsic difference in affinity of the local anesthetics for the lipid-protein interface because the membrane partition coefficients are in the order benzocaine ≫ tetracaine ≈ procaine.

Original languageEnglish
Pages (from-to)9167-9175
Number of pages9
JournalBiochemistry
Volume42
Issue number30
DOIs
StatePublished - 5 Aug 2003
Externally publishedYes

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Electric Organ
Tetracaine
Torpedo
Cholinergic Receptors
Phosphatidylinositols
Local Anesthetics
Phosphatidylcholines
Benzocaine
Procaine
Association reactions
Membranes
Lipids
Phosphatidylglycerols
Proteins
Cardiolipins
Gangliosides
Phosphatidylserines
Nicotinic Receptors
Paramagnetic resonance
Phospholipids

Cite this

Mantipragada, S. B. ; Horváth, L. I. ; Arias, H. R. ; Schwarzmann, G. ; Sandhoff, K. ; Barrantes, F. J. ; Marsh, D. / Lipid-protein interactions and effect of local anesthetics in acetylcholine receptor-rich membranes from Torpedo marmorata electric organ. In: Biochemistry. 2003 ; Vol. 42, No. 30. pp. 9167-9175.
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Mantipragada, SB, Horváth, LI, Arias, HR, Schwarzmann, G, Sandhoff, K, Barrantes, FJ & Marsh, D 2003, 'Lipid-protein interactions and effect of local anesthetics in acetylcholine receptor-rich membranes from Torpedo marmorata electric organ', Biochemistry, vol. 42, no. 30, pp. 9167-9175. https://doi.org/10.1021/bi034485q

Lipid-protein interactions and effect of local anesthetics in acetylcholine receptor-rich membranes from Torpedo marmorata electric organ. / Mantipragada, S. B.; Horváth, L. I.; Arias, H. R.; Schwarzmann, G.; Sandhoff, K.; Barrantes, F. J.; Marsh, D.

In: Biochemistry, Vol. 42, No. 30, 05.08.2003, p. 9167-9175.

Research output: Contribution to journalArticle

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T1 - Lipid-protein interactions and effect of local anesthetics in acetylcholine receptor-rich membranes from Torpedo marmorata electric organ

AU - Mantipragada, S. B.

AU - Horváth, L. I.

AU - Arias, H. R.

AU - Schwarzmann, G.

AU - Sandhoff, K.

AU - Barrantes, F. J.

AU - Marsh, D.

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N2 - The selectivity of lipid-protein interaction for spin-labeled phospholipids and gangliosides in nicotinic acetylcholine receptor-rich membranes from Torpedo marmorata has been studied by ESR spectroscopy. The association constants of the spin-labeled lipids (relative to phosphatidylcholine) at Ph 8.0 are in the order cardiolipin (5.1) ≈ stearic acid (4.9) ≈ phosphatidylinositol (4.7) > phosphatidylserine (2.7) > phosphatidylglycerol (1.7) > GD1b ≈ GM1 ≈ GM2 ≈ GM3 ≈ phosphatidylcholine (1.0) > phosphatidylethanolamine (0.5). No selectivity for mono- or disialogangliosides is found over that for phosphatidylcholine. Aminated local anesthetics were found to compete with spin-labeled phosphatidylinositol, but to a much lesser extent with spin-labeled stearic acid, for sites on the intramembranous surface of the protein. The relative association constant of phosphatidylinositol was reduced in the presence of the different local anesthetics to the following extents: tetracaine (55%) > procaine (35%) ≈ benzocaine (30%). For stearic acid, only tetracaine gave an appreciable reduction (30%) in association constant. These displacements represent an intrinsic difference in affinity of the local anesthetics for the lipid-protein interface because the membrane partition coefficients are in the order benzocaine ≫ tetracaine ≈ procaine.

AB - The selectivity of lipid-protein interaction for spin-labeled phospholipids and gangliosides in nicotinic acetylcholine receptor-rich membranes from Torpedo marmorata has been studied by ESR spectroscopy. The association constants of the spin-labeled lipids (relative to phosphatidylcholine) at Ph 8.0 are in the order cardiolipin (5.1) ≈ stearic acid (4.9) ≈ phosphatidylinositol (4.7) > phosphatidylserine (2.7) > phosphatidylglycerol (1.7) > GD1b ≈ GM1 ≈ GM2 ≈ GM3 ≈ phosphatidylcholine (1.0) > phosphatidylethanolamine (0.5). No selectivity for mono- or disialogangliosides is found over that for phosphatidylcholine. Aminated local anesthetics were found to compete with spin-labeled phosphatidylinositol, but to a much lesser extent with spin-labeled stearic acid, for sites on the intramembranous surface of the protein. The relative association constant of phosphatidylinositol was reduced in the presence of the different local anesthetics to the following extents: tetracaine (55%) > procaine (35%) ≈ benzocaine (30%). For stearic acid, only tetracaine gave an appreciable reduction (30%) in association constant. These displacements represent an intrinsic difference in affinity of the local anesthetics for the lipid-protein interface because the membrane partition coefficients are in the order benzocaine ≫ tetracaine ≈ procaine.

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