L-type calcium channels in the hippocampus and cerebellum of Alzheimer's disease brain tissue

Alexander L. Coon, David Wallace, Charles F. MacTutus, Rosemarie M. Booze

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

There is growing evidence that the selective neuronal cell death observed in Alzheimer's Disease (AD) is the result of dysregulation of intracellular calcium (Ca2+) homeostasis. In the present study, L-type voltage sensitive calcium channels (L-VSCCs) were examined in the cerebellum and hippocampus of AD (n = 6; postmortem interval less than 5 h) and age-matched control (n = 6) tissue by homogenate binding techniques and quantitative in vitro receptor autoradiography using [3H]isradipine (PN200-110). Saturation analyses of the cerebellum revealed unaltered [3H]isradipine binding parameters (K(d) and B(max)) between AD and control subjects. Analysis of AD and control hippocampus demonstrated significant differences as [3H]isradipine binding increased (62%) in AD, whereas hippocampal cell density decreased (29%) in AD, relative to control subjects. Moreover, AD differentially affected L-VSCC in area CA1 and dentate gyrus. The dentate gyrus had greatly increased binding (77%) with little cell loss (16%) in AD brains, whereas area CA1 had increased binding (40%) with significant cell loss (42%) in AD brains, relative to controls. The results of the present study suggest that hippocampal area CA1 may experience greater cell loss in response to increased L-VSCCs in AD relative to other brain regions. Copyright (C) 1999 Elsevier Science Inc.

Original languageEnglish
Pages (from-to)597-603
Number of pages7
JournalNeurobiology of Aging
Volume20
Issue number6
DOIs
StatePublished - 1 Nov 1999

Fingerprint

Cerebellar Diseases
L-Type Calcium Channels
Hippocampus
Alzheimer Disease
Brain
Isradipine
Calcium Channels
Dentate Gyrus
Cerebellum
Autoradiography
Homeostasis
Cell Death
Cell Count
Calcium

Keywords

  • Alzheimer's disease
  • CA1
  • Calcium channel
  • Dentate gyrus
  • Dihydropyridine
  • Hippocampal formation
  • Isradipine
  • Receptor autoradiography
  • Receptor binding
  • [H]PN200-110

Cite this

Coon, Alexander L. ; Wallace, David ; MacTutus, Charles F. ; Booze, Rosemarie M. / L-type calcium channels in the hippocampus and cerebellum of Alzheimer's disease brain tissue. In: Neurobiology of Aging. 1999 ; Vol. 20, No. 6. pp. 597-603.
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L-type calcium channels in the hippocampus and cerebellum of Alzheimer's disease brain tissue. / Coon, Alexander L.; Wallace, David; MacTutus, Charles F.; Booze, Rosemarie M.

In: Neurobiology of Aging, Vol. 20, No. 6, 01.11.1999, p. 597-603.

Research output: Contribution to journalArticle

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