L-type calcium channels in the hippocampus and cerebellum of Alzheimer's disease brain tissue

Alexander L. Coon, David R. Wallace, Charles F. MacTutus, Rosemarie M. Booze

Research output: Contribution to journalArticle

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Abstract

There is growing evidence that the selective neuronal cell death observed in Alzheimer's Disease (AD) is the result of dysregulation of intracellular calcium (Ca2+) homeostasis. In the present study, L-type voltage sensitive calcium channels (L-VSCCs) were examined in the cerebellum and hippocampus of AD (n = 6; postmortem interval less than 5 h) and age-matched control (n = 6) tissue by homogenate binding techniques and quantitative in vitro receptor autoradiography using [3H]isradipine (PN200-110). Saturation analyses of the cerebellum revealed unaltered [3H]isradipine binding parameters (K(d) and B(max)) between AD and control subjects. Analysis of AD and control hippocampus demonstrated significant differences as [3H]isradipine binding increased (62%) in AD, whereas hippocampal cell density decreased (29%) in AD, relative to control subjects. Moreover, AD differentially affected L-VSCC in area CA1 and dentate gyrus. The dentate gyrus had greatly increased binding (77%) with little cell loss (16%) in AD brains, whereas area CA1 had increased binding (40%) with significant cell loss (42%) in AD brains, relative to controls. The results of the present study suggest that hippocampal area CA1 may experience greater cell loss in response to increased L-VSCCs in AD relative to other brain regions. Copyright (C) 1999 Elsevier Science Inc.

Original languageEnglish
Pages (from-to)597-603
Number of pages7
JournalNeurobiology of Aging
Volume20
Issue number6
DOIs
StatePublished - 1 Nov 1999

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Keywords

  • Alzheimer's disease
  • CA1
  • Calcium channel
  • Dentate gyrus
  • Dihydropyridine
  • Hippocampal formation
  • Isradipine
  • Receptor autoradiography
  • Receptor binding
  • [H]PN200-110

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