Isoform-specific regulation of the sodium pump by α- and β-adrenergic agonists in the guinea-pig ventricle

J. Gao, R. Wymore, R. T. Wymore, Y. Wang, D. McKinnon, J. E. Dixon, R. T. Mathias, I. S. Cohen, G. J. Baldo

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

1. Guinea-pig ventricle was used in the RNase protection assays to determine which α-isoforms of the Na+-K+ pumps are present, and ventricular myocytes were used in whole cell patch clamp studies to investigate the actions of α- and β-adrenergic agonists on Na+-K+ pump current. 2. RNase protection assays showed that two isoforms of the α-subunit of the Na+-K+-ATPase are present in guinea-pig ventricle. The mRNA for the α1-isoform comprises 82% of the total pump message, the rest being the α2-isoform. 3. We have previously shown that β-adrenergic agonists affect Na+-K+ pump current (I(p) through a protein kinase A (PKA)-dependent pathway. We now show that these β-effects are targeted to the α1-isoform of the Na+-K+ pumps. 4. We have also previously shown that α-adrenergic agonists increase I(p) through a protein kinase C (PKC)-dependent pathway. We now show that these α-isoform effects are targeted to the α2-isoform of the Na+-K+ pumps. 5. These results suggest the effects of adrenergic activation on N+-K+ pump activity in the heart can be regionally specific, depending on which α-isoform of the Na+-K+ pump is expressed.

Original languageEnglish
Pages (from-to)377-383
Number of pages7
JournalJournal of Physiology
Volume516
Issue number2
DOIs
StatePublished - 15 Apr 1999

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Sodium-Potassium-Exchanging ATPase
Adrenergic Agonists
Protein Isoforms
Guinea Pigs
Ribonucleases
RNA Isoforms
Cyclic AMP-Dependent Protein Kinases
Adrenergic Agents
Muscle Cells
Protein Kinase C

Cite this

Gao, J. ; Wymore, R. ; Wymore, R. T. ; Wang, Y. ; McKinnon, D. ; Dixon, J. E. ; Mathias, R. T. ; Cohen, I. S. ; Baldo, G. J. / Isoform-specific regulation of the sodium pump by α- and β-adrenergic agonists in the guinea-pig ventricle. In: Journal of Physiology. 1999 ; Vol. 516, No. 2. pp. 377-383.
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Gao, J, Wymore, R, Wymore, RT, Wang, Y, McKinnon, D, Dixon, JE, Mathias, RT, Cohen, IS & Baldo, GJ 1999, 'Isoform-specific regulation of the sodium pump by α- and β-adrenergic agonists in the guinea-pig ventricle', Journal of Physiology, vol. 516, no. 2, pp. 377-383. https://doi.org/10.1111/j.1469-7793.1999.0377v.x

Isoform-specific regulation of the sodium pump by α- and β-adrenergic agonists in the guinea-pig ventricle. / Gao, J.; Wymore, R.; Wymore, R. T.; Wang, Y.; McKinnon, D.; Dixon, J. E.; Mathias, R. T.; Cohen, I. S.; Baldo, G. J.

In: Journal of Physiology, Vol. 516, No. 2, 15.04.1999, p. 377-383.

Research output: Contribution to journalArticle

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AU - Gao, J.

AU - Wymore, R.

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AU - Wang, Y.

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AU - Baldo, G. J.

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