Intracellular Ca 2+ and PKC activation do not inhibit Na + and water transport in rat CCD

Alexander Rouch, L. Chen, L. H. Kudo, P. D. Bell, B. C. Fowler, B. D. Corbitt, J. A. Schafer

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Experiments examined the effects of elevation of intracellular calcium concentration ([Ca 2+ ](i)) or activation of protein kinase C (PKC) on Na + and water transport in the rat cortical collecting duct (CCD). We measured the lumen-to-bath 22 Na + flux (J(l→b)), transepithelial voltage (V(T)), and water permeability (P(f)) in CCD from deoxycorticosterone (DOC)-treated rats. Ionomycin (0.5 and 1 μM) and thapsigargin (1 and 2 μM) were used to increase [Ca 2+ ](i). Phorbol 12-myristate 13-acetate (PMA; 0.3 and 1 μM) and oleoyl-acetyl-glycerol (OAG; 100 μM) were used as activators of PKC. [Ca 2+ ](i) was measured in isolated perfused tubules using the fluorescent dye fura 2. When added to the bathing solution, 220 pM arginine vasopressin (AVP) failed to affect [Ca 2+ ](i), whereas 1 μM ionomycin increased [Ca 2+ ](i) by 103 ± 15% and 2 μM thapsigargin increased [Ca 2+ ](i) by 24 ± 4%. In flux studies, neither ionomycin nor thapsigargin affected J(l→b) or P(f), although ionomycin caused marked morphological changes. Ionomycin also failed to alter either parameter in tubules from non-DOC-treated rats. Neither 100 μM OAG nor 1 μM PMA affected J(l→b) or P(f). OAG at 50 μM had no effect on V(T) or transepithelial resistance, indicating no inhibition of conductive Na + transport. We conclude that increased [Ca 2+ ](i) and PKC activation do not affect J(l→b) or P(f) in the rat CCD. These findings may account for the sustained increase in J(l→b) produced in the rat CCD by AVP.

Original languageEnglish
JournalAmerican Journal of Physiology - Renal Fluid and Electrolyte Physiology
Issue number4 34-4
Publication statusPublished - 1 Jan 1993



  • arginine vasopressin
  • cortical collecting duct
  • intracellular second messengers
  • ionomycin
  • mineralocorticoid
  • oleoyl-acetyl-glycerol
  • phorbol 12-myristate 13- acetate
  • protein kinase C
  • sodium channel
  • thapsigargin
  • vasopressin

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