Interleukin 6 indirectly induces keratinocyte migration

Randle M. Gallucci; Dusti K. Sloan; Julie M. Heck; Anne R. Murray; Sijy J. O'Dell

doi:10.1111/j.0022-202X.2004.22323.x

Interleukin 6 indirectly induces keratinocyte migration

Randle M. Gallucci, Dusti K. Sloan, Julie M. Heck, Anne R. Murray, Sijy J. O'Dell

Oklahoma State University Center for Health Sciences

Research output: Contribution to journal › Article › peer-review

132 Scopus citations

Abstract

IL-6-deficient transgenic mice (IL-6 KO) display significantly delayed cutaneous wound healing. To further elucidate the role of IL-6 in skin wound healing, epidermal keratinocyte and dermal fibroblast cells were isolated from neonatal IL-6 KO mice and treated with rmIL-6. It was found that rmIL-6 alone did not significantly modulate the proliferation or migration of cultured IL-6 KO keratinocytes. rmIL-6, however, significantly induced the migration of IL-6 KO keratinocytes (up to 5-fold) when co-cultured with dermal fibroblasts. Culture supernatants from IL-6-treated fibroblasts were also found to induce the migration of keratinocytes to a similar degree. Genomics analysis of treated fibroblasts indicated that rmIL-6 does not induce any known soluble keratinocyte migratory factors. rmIL-6 treatment of fibroblast, however, induced a rapid and sustained phosphorylation of STAT3 protein. These data indicate that IL-6 could influence wound healing by inducing keratinocyte migration through the production of a soluble fibroblast-derived factor, and its activity may be associated with STAT3 activation.

Original language	English
Pages (from-to)	764-772
Number of pages	9
Journal	Journal of Investigative Dermatology
Volume	122
Issue number	3
DOIs	https://doi.org/10.1111/j.0022-202X.2004.22323.x
State	Published - Mar 2004

Keywords

Cytokines
Epidermis
Inflammation
Wound healinq

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title = "Interleukin 6 indirectly induces keratinocyte migration",

abstract = "IL-6-deficient transgenic mice (IL-6 KO) display significantly delayed cutaneous wound healing. To further elucidate the role of IL-6 in skin wound healing, epidermal keratinocyte and dermal fibroblast cells were isolated from neonatal IL-6 KO mice and treated with rmIL-6. It was found that rmIL-6 alone did not significantly modulate the proliferation or migration of cultured IL-6 KO keratinocytes. rmIL-6, however, significantly induced the migration of IL-6 KO keratinocytes (up to 5-fold) when co-cultured with dermal fibroblasts. Culture supernatants from IL-6-treated fibroblasts were also found to induce the migration of keratinocytes to a similar degree. Genomics analysis of treated fibroblasts indicated that rmIL-6 does not induce any known soluble keratinocyte migratory factors. rmIL-6 treatment of fibroblast, however, induced a rapid and sustained phosphorylation of STAT3 protein. These data indicate that IL-6 could influence wound healing by inducing keratinocyte migration through the production of a soluble fibroblast-derived factor, and its activity may be associated with STAT3 activation.",

keywords = "Cytokines, Epidermis, Inflammation, Wound healinq",

author = "Gallucci, {Randle M.} and Sloan, {Dusti K.} and Heck, {Julie M.} and Murray, {Anne R.} and O'Dell, {Sijy J.}",

note = "Funding Information: The authors thank Dr Melanie Flint for her critical review of this manuscript. This work was supported by grant #HR01-044 from the Oklahoma Center for the Advancement of Science and Technology.",

year = "2004",

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doi = "10.1111/j.0022-202X.2004.22323.x",

language = "English",

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AU - Gallucci, Randle M.

AU - Sloan, Dusti K.

AU - Heck, Julie M.

AU - Murray, Anne R.

AU - O'Dell, Sijy J.

N1 - Funding Information: The authors thank Dr Melanie Flint for her critical review of this manuscript. This work was supported by grant #HR01-044 from the Oklahoma Center for the Advancement of Science and Technology.

PY - 2004/3

Y1 - 2004/3

N2 - IL-6-deficient transgenic mice (IL-6 KO) display significantly delayed cutaneous wound healing. To further elucidate the role of IL-6 in skin wound healing, epidermal keratinocyte and dermal fibroblast cells were isolated from neonatal IL-6 KO mice and treated with rmIL-6. It was found that rmIL-6 alone did not significantly modulate the proliferation or migration of cultured IL-6 KO keratinocytes. rmIL-6, however, significantly induced the migration of IL-6 KO keratinocytes (up to 5-fold) when co-cultured with dermal fibroblasts. Culture supernatants from IL-6-treated fibroblasts were also found to induce the migration of keratinocytes to a similar degree. Genomics analysis of treated fibroblasts indicated that rmIL-6 does not induce any known soluble keratinocyte migratory factors. rmIL-6 treatment of fibroblast, however, induced a rapid and sustained phosphorylation of STAT3 protein. These data indicate that IL-6 could influence wound healing by inducing keratinocyte migration through the production of a soluble fibroblast-derived factor, and its activity may be associated with STAT3 activation.

AB - IL-6-deficient transgenic mice (IL-6 KO) display significantly delayed cutaneous wound healing. To further elucidate the role of IL-6 in skin wound healing, epidermal keratinocyte and dermal fibroblast cells were isolated from neonatal IL-6 KO mice and treated with rmIL-6. It was found that rmIL-6 alone did not significantly modulate the proliferation or migration of cultured IL-6 KO keratinocytes. rmIL-6, however, significantly induced the migration of IL-6 KO keratinocytes (up to 5-fold) when co-cultured with dermal fibroblasts. Culture supernatants from IL-6-treated fibroblasts were also found to induce the migration of keratinocytes to a similar degree. Genomics analysis of treated fibroblasts indicated that rmIL-6 does not induce any known soluble keratinocyte migratory factors. rmIL-6 treatment of fibroblast, however, induced a rapid and sustained phosphorylation of STAT3 protein. These data indicate that IL-6 could influence wound healing by inducing keratinocyte migration through the production of a soluble fibroblast-derived factor, and its activity may be associated with STAT3 activation.

KW - Cytokines

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KW - Inflammation

KW - Wound healinq

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Interleukin 6 indirectly induces keratinocyte migration

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