Inhibition of selenite-catalyzed superoxide generation and formation of elemental selenium (Se°) by copper, zinc, and aurintricarboxylic acid (ATA)

Randall Davis, Julian E. Spallholz

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Selenite catalyzes the oxidation of glutathione (GSH) with the subsequent generation of superoxide (O2·-). Copper, zinc, and aurintricarboxylic acid (ATA) were tested for their ability to inhibit both the selenite-catalyzed generation of superoxide and the conversion of selenite to elemental selenium (Se°). As measured by lucigenin-dependent chemiluminescence (CL), copper, zinc, and ATA were shown to inhibit significantly (P < 0.05) selenite-catalyzed CL in a concentration-dependent manner. The inhibition of the selenium-catalyzed generation of superoxide by copper(II) was greater than by either zinc or ATA. In addition, copper, zinc, and ATA all inhibited the conversion of selenite to Se°. Inhibition of selenite-catalyzed CL by copper, zinc, and ATA is believed to occur as the result of inhibition of Se2- and/or GSSe-, the catalytic selenopersulfide anion of GSH.

Original languageEnglish
Pages (from-to)1015-1020
Number of pages6
JournalBiochemical Pharmacology
Volume51
Issue number8
DOIs
StatePublished - 26 Apr 1996

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Aurintricarboxylic Acid
Selenious Acid
Selenium
Superoxides
Zinc
Copper
Chemiluminescence
Luminescence
Anions
Glutathione
Oxidation

Keywords

  • aurintricarboxylic acid
  • copper
  • selenite
  • superoxide
  • toxicity
  • zinc

Cite this

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title = "Inhibition of selenite-catalyzed superoxide generation and formation of elemental selenium (Se°) by copper, zinc, and aurintricarboxylic acid (ATA)",
abstract = "Selenite catalyzes the oxidation of glutathione (GSH) with the subsequent generation of superoxide (O2·-). Copper, zinc, and aurintricarboxylic acid (ATA) were tested for their ability to inhibit both the selenite-catalyzed generation of superoxide and the conversion of selenite to elemental selenium (Se°). As measured by lucigenin-dependent chemiluminescence (CL), copper, zinc, and ATA were shown to inhibit significantly (P < 0.05) selenite-catalyzed CL in a concentration-dependent manner. The inhibition of the selenium-catalyzed generation of superoxide by copper(II) was greater than by either zinc or ATA. In addition, copper, zinc, and ATA all inhibited the conversion of selenite to Se°. Inhibition of selenite-catalyzed CL by copper, zinc, and ATA is believed to occur as the result of inhibition of Se2- and/or GSSe-, the catalytic selenopersulfide anion of GSH.",
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Inhibition of selenite-catalyzed superoxide generation and formation of elemental selenium (Se°) by copper, zinc, and aurintricarboxylic acid (ATA). / Davis, Randall; Spallholz, Julian E.

In: Biochemical Pharmacology, Vol. 51, No. 8, 26.04.1996, p. 1015-1020.

Research output: Contribution to journalArticle

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AU - Davis, Randall

AU - Spallholz, Julian E.

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N2 - Selenite catalyzes the oxidation of glutathione (GSH) with the subsequent generation of superoxide (O2·-). Copper, zinc, and aurintricarboxylic acid (ATA) were tested for their ability to inhibit both the selenite-catalyzed generation of superoxide and the conversion of selenite to elemental selenium (Se°). As measured by lucigenin-dependent chemiluminescence (CL), copper, zinc, and ATA were shown to inhibit significantly (P < 0.05) selenite-catalyzed CL in a concentration-dependent manner. The inhibition of the selenium-catalyzed generation of superoxide by copper(II) was greater than by either zinc or ATA. In addition, copper, zinc, and ATA all inhibited the conversion of selenite to Se°. Inhibition of selenite-catalyzed CL by copper, zinc, and ATA is believed to occur as the result of inhibition of Se2- and/or GSSe-, the catalytic selenopersulfide anion of GSH.

AB - Selenite catalyzes the oxidation of glutathione (GSH) with the subsequent generation of superoxide (O2·-). Copper, zinc, and aurintricarboxylic acid (ATA) were tested for their ability to inhibit both the selenite-catalyzed generation of superoxide and the conversion of selenite to elemental selenium (Se°). As measured by lucigenin-dependent chemiluminescence (CL), copper, zinc, and ATA were shown to inhibit significantly (P < 0.05) selenite-catalyzed CL in a concentration-dependent manner. The inhibition of the selenium-catalyzed generation of superoxide by copper(II) was greater than by either zinc or ATA. In addition, copper, zinc, and ATA all inhibited the conversion of selenite to Se°. Inhibition of selenite-catalyzed CL by copper, zinc, and ATA is believed to occur as the result of inhibition of Se2- and/or GSSe-, the catalytic selenopersulfide anion of GSH.

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