Influence of cell surface hydrophobicity properties on susceptivity to Triclosan sensitization by outer membrane permeabilization in opportunistically pathogenic Serratia species.

A. Rigsbee, A. Benton, B. King, W. Sprinkles, S. Katz-Amburn, Franklin Champlin

Research output: Contribution to conferencePoster

Abstract

Unlike most hydrophobic molecules, the biocide triclosan is able to penetrate the gram-negative bacterial outer membrane. The nosocomial opportunists Pseudomonas aeruginosa and Serratia marcesens are typically resistant to triclosan due largely to outer membrane impermeability properties for hydrophobic substances. However, we have recently shown that the degree of outer membrane involvement differs among disparate opportunistically pathogenic Serratia species. Cell surface hydrophobicity (CSH) properties underlie susceptivity to triclosan sensitization by outer membrane permeabilization in opportunistic Serratia species. A model system consisting of opportunistic Serratia species (marcesens, fonticola, and odorifera) exhibiting disparate degrees of susceptivity to hydrophobic antibacterial agents and triclosansensitization by outer membrane permeabilizer compound 48/80 were examined. Overall susceptibility to disparate hydrophobic antibacterial agents was determined using conventional disc agar diffusion and macrobroth dilution bioassays. Batch culture kinetics with triclosan and outer membrane permeabilizer compound 48/80 allowed analysis of cell envelope involvement in intrinsic triclosan resistance. CSH was determined using the hydrocarbon adherence method. S. marcesens and S. fonticola intrinsically resistant to hydrophobic antibacterial agents including triclosan, while S. odorifera was susceptible. CSH properties differed only slightly compared with control organisms, regardless of how susceptive they were to triclosan sensitization. These data suggest that the phenotypic differences seen in three opportunistic Serratia species with regard to intrinsic resistance to triclosan are at least partly due to disparate outer membrane exclusion potential. Moreover, susceptivity to triclosan sensitization by outer membrane permeabilization appears not to be influenced by CSH properties.
Original languageAmerican English
StatePublished - 22 Aug 2020
EventOklahoma State University Center for Health Sciences Research Day 2019 - Oklahoma State University Center for Health Sciences, TULSA, United States
Duration: 21 Feb 201922 Feb 2019
https://hdl.handle.net/11244/323882

Conference

ConferenceOklahoma State University Center for Health Sciences Research Day 2019
Abbreviated titleResearch Day 2019
CountryUnited States
CityTULSA
Period21/02/1922/02/19
Internet address

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    Rigsbee, A., Benton, A., King, B., Sprinkles, W., Katz-Amburn, S., & Champlin, F. (2020). Influence of cell surface hydrophobicity properties on susceptivity to Triclosan sensitization by outer membrane permeabilization in opportunistically pathogenic Serratia species.. Poster session presented at Oklahoma State University Center for Health Sciences Research Day 2019, TULSA, United States. https://shareok.org/handle/11244/323863