Indomethacin and staurosporine reverse α2 inhibition of water transport in rat IMCD

Alexander Rouch, Lúcia H. Kudo

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

These studies were conducted to determine if the prostaglandin-synthesis inhibitor indomethacin or the protein kinase C (PKC) inhibitor staurosporine affect the inhibition of osmotic water permeability (P(f)) by the alpha-2 (α2) agonist dexmedetomidine in the rat inner medullary collecting duct (IMCD). Terminal IMCDs from Wistar rats were perfused and P(f) was increased with either 220 pM arginine vasopressin (AVP) or 0.1 mM 8-chlorophenylthio cyclic adenosine monophosphate (8CPTcAMP). All agents were added to the bathing solution. Dexmedetomidine at 100 nM inhibited both AVP- and 8CPTcAMP- stimulated P(f). When P(f) was increased by AVP, indomethacin at 0.1 mM or 5 μM reversed the dexmedetomidine-induced inhibition by 68% and 43%. respectively. When P(f) was increased by 8CPTcAMP, indomethacin at 0.1 mu or 5 μM reversed inhibition by 83% and 70%, respectively. Indomethacin increased AVP and 8CPTcAMP-stimulated P(f) by 20 to 30% and dexmedetomidine inhibited the AVP+ indomethacin-stimulated P(f). Staurosporine at 10 nM yielded similar results. Results suggest that PKC and prostaglandins are involved in the α2 mediated mechanism, and staurosporine and indomethacin- sensitive cellular mediators modulate basal P(f).

Original languageEnglish
Pages (from-to)1351-1358
Number of pages8
JournalKidney International
Volume52
Issue number5
DOIs
StatePublished - 1 Jan 1997

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Staurosporine
Arginine Vasopressin
Indomethacin
Dexmedetomidine
Cyclic AMP
Water
Protein Kinase C
Prostaglandin Antagonists
Protein C Inhibitor
Protein Kinase Inhibitors
Prostaglandins
Wistar Rats
Permeability

Cite this

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title = "Indomethacin and staurosporine reverse α2 inhibition of water transport in rat IMCD",
abstract = "These studies were conducted to determine if the prostaglandin-synthesis inhibitor indomethacin or the protein kinase C (PKC) inhibitor staurosporine affect the inhibition of osmotic water permeability (P(f)) by the alpha-2 (α2) agonist dexmedetomidine in the rat inner medullary collecting duct (IMCD). Terminal IMCDs from Wistar rats were perfused and P(f) was increased with either 220 pM arginine vasopressin (AVP) or 0.1 mM 8-chlorophenylthio cyclic adenosine monophosphate (8CPTcAMP). All agents were added to the bathing solution. Dexmedetomidine at 100 nM inhibited both AVP- and 8CPTcAMP- stimulated P(f). When P(f) was increased by AVP, indomethacin at 0.1 mM or 5 μM reversed the dexmedetomidine-induced inhibition by 68{\%} and 43{\%}. respectively. When P(f) was increased by 8CPTcAMP, indomethacin at 0.1 mu or 5 μM reversed inhibition by 83{\%} and 70{\%}, respectively. Indomethacin increased AVP and 8CPTcAMP-stimulated P(f) by 20 to 30{\%} and dexmedetomidine inhibited the AVP+ indomethacin-stimulated P(f). Staurosporine at 10 nM yielded similar results. Results suggest that PKC and prostaglandins are involved in the α2 mediated mechanism, and staurosporine and indomethacin- sensitive cellular mediators modulate basal P(f).",
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Indomethacin and staurosporine reverse α2 inhibition of water transport in rat IMCD. / Rouch, Alexander; Kudo, Lúcia H.

In: Kidney International, Vol. 52, No. 5, 01.01.1997, p. 1351-1358.

Research output: Contribution to journalArticle

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