Identification Through DNA Analysis in Criminal and Family-Relatedness Investigations

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

This chapter discusses the identification through DNA analysis in criminal and family-relatedness investigations. Genetic polymorphisms in chromosomal DNA revolutionized the field of identification testing and radically improved the level of certainty in conclusions issued by labs performing parentage or forensic testing. The variability in DNA discovered were associated with the human myoglobin gene. DNA "fingerprints" could be visualized in human genomic DNA using the then-rather-standard molecular technique known as restriction fragment length polymorphism analysis. The highly variable DNA markers visualized through RFLP analysis group exhibited a molecular structure consisting of a short sequence of nucleotides that was tandemly repeated a variable number of times along a length of chromosome. If chromosomal DNA is digested with a restriction enzyme that cuts in the DNA flanking the tandem array, or if polymerase chain reaction (PCR) amplification is performed using primers designed to recognize sites in the flanking DNA, products will be produced whose length is proportional to the number of repeats in the array. Electrophoresis can then be used to separate and, in the case of RFLP analysis, a Southern blot hybridized under stringent conditions to a complementary labeled DNA probe will allow the VNTR alleles in the DNA sample to be visualized. If the VNTR alleles are produced using PCR, allelic products will accumulate to levels that can be easily visualized using generic DNA stains or through modification of one or both primers used to direct the amplification. © 2010

Original languageEnglish
Title of host publicationMolecular Diagnostics
PublisherElsevier Inc.
Pages381-398
Number of pages18
ISBN (Print)9780123694287
DOIs
StatePublished - 1 Dec 2010

Fingerprint

DNA
Restriction Fragment Length Polymorphisms
Polymerase chain reaction
Polymorphism
Alleles
Amplification
Polymerase Chain Reaction
DNA Fingerprinting
Myoglobin
DNA Probes
Genetic Polymorphisms
Southern Blotting
Molecular Structure
Genetic Markers
Testing
Electrophoresis
Chromosomes
Reaction products
Coloring Agents
Complementary DNA

Cite this

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Identification Through DNA Analysis in Criminal and Family-Relatedness Investigations. / Allen, Robert.

Molecular Diagnostics. Elsevier Inc., 2010. p. 381-398.

Research output: Chapter in Book/Report/Conference proceedingChapter

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