Identification of cis-regulatory regions necessary for robust Nos2 promoter activity in glial cells: Indirect role for NF-κB

Alma C. Sanchez, Randall Davis, Peter J. Syapin

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Previous reports suggest the nitric-oxide synthase 2 (Nos2) promoter contains negative and positive cis-regulatory regions. This study identified such regions using rat C6 glial cells. Activity of the serially deleted rat Nos2 promoter fused to a luciferase reporter gene was found to vary with construct size independent of stimuli, decreasing markedly from 160 to 130 bp then increasing significantly from 110 to 94 bp. In contrast, time to peak activity was stimulus-dependent but size-independent; 4-8 h for a cytokine mixture or lipopolysaccharide + interferon-γ, and 8-16 h for lipopolysaccharide + phorbol 12-myristate 13-acetate. Peak activity with heterologous promoters also varied; 4 h for 3.7 kb of the human Nos2A promoter, and 36 h for 1.8 kb of the murine promoter. Electrophoretic mobility shift assays and in vivo DNA footprinting data confirmed nuclear protein binding to promoter regions suspected of containing important regulatory sites based on reporter gene data. A binding site for NF-κB was not required for Nos2 promoter activity. These findings provide significant new information on the relative importance of different regions of the rat Nos2 promoter for transcriptional activation and nitric oxide production by glial cells and support the existence of cell- and species-specific mechanisms for transcriptional regulation of Nos2 activation.

Original languageEnglish
Pages (from-to)1379-1390
Number of pages12
JournalJournal of Neurochemistry
Volume86
Issue number6
DOIs
StatePublished - 1 Sep 2003

Fingerprint

Nucleic Acid Regulatory Sequences
Nitric Oxide Synthase
Neuroglia
Rats
Reporter Genes
Lipopolysaccharides
DNA Footprinting
Genes
Chemical activation
Electrophoretic mobility
Electrophoretic Mobility Shift Assay
Nuclear Proteins
Luciferases
Genetic Promoter Regions
Protein Binding
Interferons
Transcriptional Activation
Assays
Nitric Oxide
Acetates

Keywords

  • Astrocyte
  • Gene regulation
  • Nitric oxide
  • Protein-DNA interactions
  • iNOS

Cite this

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abstract = "Previous reports suggest the nitric-oxide synthase 2 (Nos2) promoter contains negative and positive cis-regulatory regions. This study identified such regions using rat C6 glial cells. Activity of the serially deleted rat Nos2 promoter fused to a luciferase reporter gene was found to vary with construct size independent of stimuli, decreasing markedly from 160 to 130 bp then increasing significantly from 110 to 94 bp. In contrast, time to peak activity was stimulus-dependent but size-independent; 4-8 h for a cytokine mixture or lipopolysaccharide + interferon-γ, and 8-16 h for lipopolysaccharide + phorbol 12-myristate 13-acetate. Peak activity with heterologous promoters also varied; 4 h for 3.7 kb of the human Nos2A promoter, and 36 h for 1.8 kb of the murine promoter. Electrophoretic mobility shift assays and in vivo DNA footprinting data confirmed nuclear protein binding to promoter regions suspected of containing important regulatory sites based on reporter gene data. A binding site for NF-κB was not required for Nos2 promoter activity. These findings provide significant new information on the relative importance of different regions of the rat Nos2 promoter for transcriptional activation and nitric oxide production by glial cells and support the existence of cell- and species-specific mechanisms for transcriptional regulation of Nos2 activation.",
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Identification of cis-regulatory regions necessary for robust Nos2 promoter activity in glial cells : Indirect role for NF-κB. / Sanchez, Alma C.; Davis, Randall; Syapin, Peter J.

In: Journal of Neurochemistry, Vol. 86, No. 6, 01.09.2003, p. 1379-1390.

Research output: Contribution to journalArticle

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