Hydrophilic diol monolith for the preparation of immuno-sorbents at reduced nonspecific interactions

Dilani N. Gunasena, Ziad El Rassi

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

A polar organic polymer monolith (M1) was introduced for performing immunoaffinity chromatography (IAC) at reduced nonspecific interactions. The M1 monolith was prepared by the in situ polymerization of glyceryl methacrylate (GMM) and pentaerythritol triacrylate (PETA). Through its surface diol groups, M1 provided the functionalities to immobilize antibodies. Anti-haptoglobin antibody was used as the model antibody to study the overall behavior of the immuno monolith M1 in terms of its binding to the antigen and to evaluate its nonspecific binding with other proteins, especially the high-abundance human serum proteins. To better assess the suitability of M1 for IAC, other immuno monoliths were prepared and compared with the immuno monolith M1. Two monoliths were of the traditional ones: copolymers of (i) glycidyl methacrylate and ethylene glycol dimethacrylate (EDMA) and (ii) GMM and EDMA, referred to as M2 and M3, respectively. A fourth monolith involving the copolymerization of N-(3-aminopropyl)methacrylamide hydrochloride and EDMA (M4) was introduced to allow the site-directed immobilization of antibodies. Owing to its hydroxyl groups, the M1 exhibited negligible nonspecific hydrophobic interactions with proteins. On the other hand, M4 exhibited extensive electrostatic interactions, while the M2 and to a lesser extent M3 exhibited hydrophobic interactions.

Original languageEnglish
Pages (from-to)2097-2105
Number of pages9
JournalJournal of Separation Science
Volume34
Issue number16-17
DOIs
StatePublished - 1 Aug 2011
Externally publishedYes

Keywords

  • Human serum
  • Immuno monolithic columns
  • Immunoaffinity chromatography
  • Nonspecific binding

Fingerprint Dive into the research topics of 'Hydrophilic diol monolith for the preparation of immuno-sorbents at reduced nonspecific interactions'. Together they form a unique fingerprint.

  • Cite this