Our previous work has shown the presence of histamine releasing factors in a variety of snake venoms. To further study the occurrence of such factors, a three method isolation approach was used. A peptide fraction of the venom of C. v. helleri was prepared by ultrafiltration. This 10KD filtrate was analyzed by cation exchange chromatography. The filtrate and three pools of fractions from the cation exchange experiment were analyzed by HPLC reversed phase chromatography. When examining the elution profiles, the pools tended to separate in HPLC-RPC into two clusters of eluting peaks. Multiple peaks of histamine releasing activity were found when the filtrate was separated by either chromatographic method. The bioassay appeared very sensitive in detecting factors of interest, could be related to the elution profiles, and at times found peaks of activity without their visualization in the elution profiles. These observations indicate that C.v. helleri venom contains a variety of histamine releasing activities amenable to study by cation exchange and HPLC-RP chromatography, that the peptide fraction of at least this venom would be proinflammatory and contribute to the pathology of envenomation, and provides a useful system with which to study the effects on mast cells of naturally occurring peptides and the peptidergic pathway.
|Number of pages||10|
|Journal||Research Communications in Pharmacology and Toxicology|
|Publication status||Published - 1 Jan 1998|