Green synthesis of a new gelatin-based antimicrobial scaffold for tissue engineering

Mostafa Yazdimamaghani, Daryoosh Vashaee, Senait Assefa, Mitra Shabrangharehdasht, Armin Tahmasbi Rad, Margaret A. Eastman, Kenneth J. Walker, Sundar V. Madihally, Gerwald A. Köhler, Lobat Tayebi

Research output: Contribution to journalArticlepeer-review

42 Scopus citations


With the aim of developing appropriate scaffolds for tissue engineering to suppress the formation of biofilms, an effective one-pot process was applied in this study to produce scaffolds with inherent antibacterial activity. A new method to synthesize genipin-crosslinked gelatin/nanosilver scaffolds with "green" in situ formation of silver nanoparticles by heat treatment is presented in this paper. In this procedure, toxic solvents, reducing agents, and stabilizing agents are avoided. UV-visible absorption spectra of the synthesized gelatin/nanosilver solutions were obtained immediately and three months after the synthesis revealing the presence and high stability of the silver nanoparticles. The TEM of gelatin/nanosilver solutions showed silver particles with spherical shapes that were less than 5 nm in size. Interestingly, contact angle was found to increase from 80 to 125 with the increase in concentration of nanosilver in gelatin. All gelatin/nanosilver solutions showed antimicrobial activity against Staphylococcus aureus and Escherichia coli. However, only the highest concentration showed antifungal effects against Candida albicans pathogens. Scaffolds were prepared by a lyophilization technique from this solution and their antimicrobial activities were examined. Introducing this facile green one-pot process of synthesizing scaffolds with antimicrobial and anti-biofilm properties may lead to key applications in tissue engineering techniques.

Original languageEnglish
Pages (from-to)235-244
Number of pages10
JournalMaterials Science and Engineering C
Issue number1
StatePublished - 1 Jun 2014


  • Antibacterial activity
  • Biofilm
  • Scaffold
  • Silver nanoparticle
  • Tissue engineering


Dive into the research topics of 'Green synthesis of a new gelatin-based antimicrobial scaffold for tissue engineering'. Together they form a unique fingerprint.

Cite this