Glutamine synthetase activities in spinal white and gray matter 7 days following spinal cord injury in rats

Richard L. Benton, C. David Ross, Kenneth Miller

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The glial enzyme glutamine synthetase (GS) is critical for central nervous system catabolism of glutamate and glutamine production. Upregulation of GS is a hallmark of reactive astrocytosis, although such induction following spinal cord injury (SCI) has not been reported. This study's purpose was to determine if GS activity is increased following SCI. Experimental rats received a complete spinal transection at the T5 segment and control rats received a laminectomy only. GS activities were determined using an enzymatic microassay. Glutamine levels were resolved in semi- adjacent sections. At 7 days following SCI, GS activity increased an average of 170-190% in white matter and 15-25% in gray matter immediately adjacent to the transection, and 70-90% in white matter and 40-45% in gray matter from cervical and lumbar enlargements. Correlative increases in glutamine were observed also. These findings further characterize the astrocytic response to SCI, which may contribute to altered glutamine metabolism in injured spinal tissue. (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)1-4
Number of pages4
JournalNeuroscience Letters
Volume291
Issue number1
DOIs
StatePublished - 8 Sep 2000

Fingerprint

Glutamate-Ammonia Ligase
Spinal Cord Injuries
Glutamine
Gliosis
Laminectomy
Neuroglia
Glutamic Acid
Up-Regulation
Central Nervous System
Gray Matter
White Matter
Enzymes

Keywords

  • Astrocyte
  • Glutamine
  • Glutamine synthetase
  • Injury
  • Rat
  • Spinal cord

Cite this

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Glutamine synthetase activities in spinal white and gray matter 7 days following spinal cord injury in rats. / Benton, Richard L.; Ross, C. David; Miller, Kenneth.

In: Neuroscience Letters, Vol. 291, No. 1, 08.09.2000, p. 1-4.

Research output: Contribution to journalArticle

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