Glutaminase immunoreactivity and enzyme activity is increased in the rat dorsal root ganglion following peripheral inflammation

Kenneth Miller, John C. Balbás, Richard L. Benton, Travis S. Lam, Kristin M. Edwards, Richard M. Kriebel, Ruben Schechter

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Following inflammation, primary sensory neurons in the dorsal root ganglion (DRG) alter the production of several proteins.Most DRG neurons are glutamatergic, using glutaminase as the enzyme for glutamate production, but little is known about glutaminase following inflammation. In the present study, adjuvant-induced arthritis (AIA) was produced in rats with complete Freund's adjuvant into the hindpaw. At 7 days of AIA, DRG were examined with glutaminase immunohistochemistry,Western blot immunoreactivity, and enzyme activity. Image analysis revealed that glutaminase was elevated most in small-sized neurons (21%) (P < 0.05). Western blot analysis revealed a 19% increase (P < 0.05) in total glutaminase and 21% in mitochondrial glutaminase (P < 0.05). Glutaminase enzyme activity was elevated 29% (P < 0.001) from 2.20 to 2.83 moles/kg/hr. Elevated glutaminase in primary sensory neurons could lead to increased glutamate production in spinal primary afferent terminals contributing to centra sensitization or in the peripheral process contributing to peripheral sensitization.

Original languageEnglish
Article number414697
JournalPain Research and Treatment
Volume2012
DOIs
StatePublished - 1 Dec 2012

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Glutaminase
Spinal Ganglia
Inflammation
Enzymes
Experimental Arthritis
Sensory Receptor Cells
Glutamic Acid
Western Blotting
Neurons
Freund's Adjuvant
Immunohistochemistry

Cite this

Miller, Kenneth ; Balbás, John C. ; Benton, Richard L. ; Lam, Travis S. ; Edwards, Kristin M. ; Kriebel, Richard M. ; Schechter, Ruben. / Glutaminase immunoreactivity and enzyme activity is increased in the rat dorsal root ganglion following peripheral inflammation. In: Pain Research and Treatment. 2012 ; Vol. 2012.
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abstract = "Following inflammation, primary sensory neurons in the dorsal root ganglion (DRG) alter the production of several proteins.Most DRG neurons are glutamatergic, using glutaminase as the enzyme for glutamate production, but little is known about glutaminase following inflammation. In the present study, adjuvant-induced arthritis (AIA) was produced in rats with complete Freund's adjuvant into the hindpaw. At 7 days of AIA, DRG were examined with glutaminase immunohistochemistry,Western blot immunoreactivity, and enzyme activity. Image analysis revealed that glutaminase was elevated most in small-sized neurons (21{\%}) (P < 0.05). Western blot analysis revealed a 19{\%} increase (P < 0.05) in total glutaminase and 21{\%} in mitochondrial glutaminase (P < 0.05). Glutaminase enzyme activity was elevated 29{\%} (P < 0.001) from 2.20 to 2.83 moles/kg/hr. Elevated glutaminase in primary sensory neurons could lead to increased glutamate production in spinal primary afferent terminals contributing to centra sensitization or in the peripheral process contributing to peripheral sensitization.",
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Glutaminase immunoreactivity and enzyme activity is increased in the rat dorsal root ganglion following peripheral inflammation. / Miller, Kenneth; Balbás, John C.; Benton, Richard L.; Lam, Travis S.; Edwards, Kristin M.; Kriebel, Richard M.; Schechter, Ruben.

In: Pain Research and Treatment, Vol. 2012, 414697, 01.12.2012.

Research output: Contribution to journalArticle

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