Glutamate and metabotropic glutamate receptors associated with innervation of the uterine cervix during pregnancy: Receptor antagonism inhibits c-Fos expression in rat lumbosacral spinal cord at parturition

Chaitali Ghosh, Megan Storey-Workley, Sharon Usip, Jen Hafemeister, Kenneth Miller, Raymond E. Papka

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Dorsal root ganglia (DRG) neurons connect the spinal cord and uterine cervix, and are activated at parturition with subsequent stimulation of secondary neurons in the spinal dorsal horn and autonomic areas. Neuropeptide neurotransmitters and receptors have been studied in these areas, but amino acid transmitters, e.g., glutamate, an excitatory neurotransmitter involved in sensory and nociceptive processing, have not been characterized. To determine if glutamate is involved in innervation of the cervix, rats were examined for markers of glutamatergic neurons in the L6-S1 spinal cord, DRG and cervix. Metabotropic glutamate receptors mGluR5 in the spinal dorsal horn and their expression over pregnancy were examined in pregnant rats and pregnant rats treated continuously with an antagonist of mGluR5, 2-methyl-6-(phenylethynyl) pyridine (MPEP). Rats were allowed to deliver pups to determine if the antagonist altered the expression of an early response gene protein, Fos, in the L6-S1 cord. Immunohistochemistry showed glutamate- and vesicular glutamate transporter1 (VGluT1)-positive fibers in the cervix, glutamate- and VGluT1-expressing neurons in the DRG, some of which also exhibited retrograde tracer from cervical injections, and VGluT1 and mGluR5 immunoreactivities in the L6-S1 spinal dorsal horns. Expression of mGluR5 receptors increased over pregnancy. Fos-positive neurons were present among mGluR5-immunoreactivity in the spinal dorsal horn. Parturition-induced Fos-positive neurons in the spinal cords were abundant in control rats, but were reduced by 70% in MPEP-treated animals. These results suggest that glutamate is likely involved in the transmission of sensory signals, possibly pain, from the cervix to the spinal cord at parturition.

Original languageEnglish
Pages (from-to)1318-1335
Number of pages18
JournalJournal of Neuroscience Research
Volume85
Issue number6
DOIs
StatePublished - 1 May 2007

Fingerprint

Metabotropic Glutamate Receptors
Cervix Uteri
Glutamic Acid
Spinal Cord
Parturition
Pregnancy
Neurons
Spinal Ganglia
Neuropeptide Receptors
Neurotransmitter Receptor
Neurotransmitter Agents
Immunohistochemistry
Amino Acids
Pain
Injections
Spinal Cord Dorsal Horn

Keywords

  • Dorsal root ganglia
  • Glutamate
  • MPEP
  • Metabotropic receptors
  • Parturition

Cite this

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title = "Glutamate and metabotropic glutamate receptors associated with innervation of the uterine cervix during pregnancy: Receptor antagonism inhibits c-Fos expression in rat lumbosacral spinal cord at parturition",
abstract = "Dorsal root ganglia (DRG) neurons connect the spinal cord and uterine cervix, and are activated at parturition with subsequent stimulation of secondary neurons in the spinal dorsal horn and autonomic areas. Neuropeptide neurotransmitters and receptors have been studied in these areas, but amino acid transmitters, e.g., glutamate, an excitatory neurotransmitter involved in sensory and nociceptive processing, have not been characterized. To determine if glutamate is involved in innervation of the cervix, rats were examined for markers of glutamatergic neurons in the L6-S1 spinal cord, DRG and cervix. Metabotropic glutamate receptors mGluR5 in the spinal dorsal horn and their expression over pregnancy were examined in pregnant rats and pregnant rats treated continuously with an antagonist of mGluR5, 2-methyl-6-(phenylethynyl) pyridine (MPEP). Rats were allowed to deliver pups to determine if the antagonist altered the expression of an early response gene protein, Fos, in the L6-S1 cord. Immunohistochemistry showed glutamate- and vesicular glutamate transporter1 (VGluT1)-positive fibers in the cervix, glutamate- and VGluT1-expressing neurons in the DRG, some of which also exhibited retrograde tracer from cervical injections, and VGluT1 and mGluR5 immunoreactivities in the L6-S1 spinal dorsal horns. Expression of mGluR5 receptors increased over pregnancy. Fos-positive neurons were present among mGluR5-immunoreactivity in the spinal dorsal horn. Parturition-induced Fos-positive neurons in the spinal cords were abundant in control rats, but were reduced by 70{\%} in MPEP-treated animals. These results suggest that glutamate is likely involved in the transmission of sensory signals, possibly pain, from the cervix to the spinal cord at parturition.",
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Glutamate and metabotropic glutamate receptors associated with innervation of the uterine cervix during pregnancy : Receptor antagonism inhibits c-Fos expression in rat lumbosacral spinal cord at parturition. / Ghosh, Chaitali; Storey-Workley, Megan; Usip, Sharon; Hafemeister, Jen; Miller, Kenneth; Papka, Raymond E.

In: Journal of Neuroscience Research, Vol. 85, No. 6, 01.05.2007, p. 1318-1335.

Research output: Contribution to journalArticle

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